DRR1与COMMD1基因在人肾上皮细胞HEK293T中相互作用的研究  

Interaction Between DRR1 and COMMD1 in Human Renal Epithelial Cell Line HEK293T

作　　者：陈潞萍 刘宇希 蔡云龙 陈晓 孙晓雨 卢方晋 CHEN Luping;LIU Yuxi;CAI Yunlong;CHEN Xiao;SUN Xiaoyu;LU Fangjin(Basic Medical School,Shenyang Medical College,Liaoning Shenyang 110034,China;School of Pharmacy,Shenyang Medical College,Liaoning Shenyang 110034,China)

机构地区：[1]沈阳医学院基础医学院,辽宁沈阳110034 [2]沈阳医学院药学院,辽宁沈阳110034

出　　处：《中国继续医学教育》2021年第27期192-195,共4页China Continuing Medical Education

基　　金：辽宁省科学技术计划项目(自然基金指导计划)(2019-ZD-0323);。

摘　　要：目的到目前为止,肿瘤抑制基因DRR1被发现在各种组织中表达广泛,但其作用机制尚未完全揭示。本研究主要对DRR1在细胞核内与COMMD1基因之间相互的作用进行研究。方法采用免疫荧光染色的方法检测DRR1和COMMD1在HEK293T细胞核内表达情况,同时,在细胞中过表达DRR1后检测细胞核内COMMD1含量变化。结果DRR1主要在细胞核内表达,且DRR1与COMMD1在细胞核内相互结合;过表达DRR1可以促进细胞核内COMMD1的富集。结论DRR1对COMMD1在细胞核内富集具有调控作用,为DRR1的作用机制提供了新的理论依据。Objective So far,tumor suppressor gene DRR1 has been found to be widely expressed in various tissues,but its mechanism of action has not been fully revealed.In this study,the interaction between DRR1 and COMMD1 gene in nucleus was researched.Methods We determined the nuclear location of DRR1 and COMMD1 in HEK293T cells by using immunofluorescence staining.We also detected the nuclear localization of COMMD1 after DRR1 overexpression.Results DRR1 was expressed in the nucleus,and co-localization with COMMD1 in the nucleus.Nuclear COMMD1 was increased after the overexpression of DRR1 in the cells.Conclusion DRR1 promotes the nuclear enrichment of COMMD1,and it would provide a new theoretical basis for the mechanism of DRR1.

关 键 词：DRR1 COMMD1 细胞核 过表达 HEK293T细胞系 免疫荧光染色 

分 类 号：R394[医药卫生—医学遗传学]