思茅藤不同极性部分化学成分分析及其免疫抑制活性  

Chemical composition and immunosuppressive activity of different fractions from Epigynum auritum

作　　者：程桂广[1] 张静[1] 杨美莲[1] 李俊华 陈婷 赵天瑞[1] 曹建新[1] 开国银 CHENG Gui-guang;ZHANG Jing;YANG Mei-lian;LI Jun-hua;CHEN Ting;ZHAO Tian-rui;CAO Jian-xin;KAI Guo-yin(Faculty of Food Science and Engineering,Kunming University of Science and Technology,Kunming 650500,China;Yunnan Xicao Biological Technology Development Co.,LTD,Kunming 650500,China;College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]昆明理工大学食品科学与工程学院,云南昆明650500 [2]云南西草生物科技开发有限公司,云南昆明650500 [3]浙江中医药大学药学院,浙江杭州310053

出　　处：《云南民族大学学报（自然科学版）》2021年第5期441-448,共8页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(32060542);云南省重大科技计划项目(2019ZF010);浙江省“万人计划”科技创新领军人才(2018R52050).

摘　　要：探究了思茅藤提取物不同极性部分的主要化学成分及其免疫抑制活性情况,并对其可能的活性机制进行初步探讨.利用D101大孔吸附树脂对思茅藤提取物不同极性部分进行划分,采用超高效液相色谱-高分辨质谱联用技术(UHPLC-MS/MS)分析各提取物的主要化学成分,并建立刀豆蛋白A(Con A)和脂多糖(LPS)刺激的小鼠脾淋巴细胞模型,通过测定不同极性部分的思茅藤提取物对小鼠脾淋巴细胞增殖及相关细胞因子分泌的影响评价其免疫抑制活性.结果显示,思茅藤提取物Fr.20%~40%段的化学成分主要为酚类物质,而Fr.80%~100%段富含以甾体类化合物为主的成分.Fr.100%部分能有效的减少Con A刺激的过量炎症因子的产生,显示出最强的免疫抑制活性,且该抑制效果主要发生在G0/G1期.This study aimed to comparatively investigate the Chemical composition and immunosuppressive activity of different fractions from Epigynum auritum and its mechanism.The E.auritum extract(EAE)was fractionated by column chromatography with macroporous resin D101.UHPLC-MS/MS was used to analyze the chemical composition of different fractions of EAE.The immunosuppressive effect of different fractions of EAE were evaluated on Con A and LPS-induced mice spleen cells,and the cell proliferation and contents TNF-αand IL-6 were recorded.The major chemical composition of Fr.20%~40%of EAE are polyphenols,while the Fr.80%~100%fractions are rich in steroidal compounds.Fr.100%showed the strongest immunosuppressive effect,inhibited the productions of TNF-a and IL-6 in Con A induced T cells and the cell cycle arrested in in the G0/G1 phase.

关 键 词：思茅藤 超高效液相色谱-高分辨质谱联用技术 化学成分 免疫抑制 

分 类 号：R284.1[医药卫生—中药学]