水溶性富勒烯C_(60)对肝细胞脂肪变性的改善效果研究  

The Improvement Effect of Water-Soluble Fullerene C_(60) on Steatotic Hepatocytes

作　　者：苏强 孙娜 程燕[1] 汪淑琴 俞梅兰[2] 张勇[3] 陈侃 SU Qiang;SUN Na;CHENG Yan;WANG Shuqin;YU Meilan;ZHANG Yong;CHEN Kan(The Key Laboratory of Biotechnology&Bioengineering(KLBB)of the State Ethnic Affairs Commission,Northwest Minzu University,Lanzhou 730030,China;College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China;The 940th Hospital of Joint Logistics Support of Chinese People’s Liberation Army Stationed in the First,Outpatient Department,Lanzhou 730050,China)

机构地区：[1]西北民族大学,生物医学研究中心生物工程与技术国家民委重点实验室,兰州730030 [2]浙江理工大学生命科学与医药学院,杭州310018 [3]中国人民解放军联勤保障部队第九四O医院,第一派驻门诊部,兰州730050

出　　处：《中国细胞生物学学报》2021年第8期1588-1595,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81972281);教育部动物医学生物工程创新团队(批准号:IRT_17R88);西北民族大学引进人才科研项目(批准号:Z20055)资助的课题。

摘　　要：该文研究水溶性富勒烯C_(60)在体外对肝细胞脂肪变性的改善效果。首先利用游离脂肪酸诱导Huh6和WRL68细胞,建立肝细胞脂肪变的体外模型,再用水溶性富勒烯C_(60)干预脂肪变细胞,利用尼罗红染色法检测胞内的脂质蓄积程度,通过检测细胞内甘油三酯(TG)的含量、细胞活性氧(ROS)水平、超氧化物歧化酶(SOD)活力、还原性谷胱甘肽(GSH)和三磷酸腺苷(ATP)含量变化,比较C_(60)的作用效果。结果显示,游离脂肪酸诱导使得细胞蓄积脂滴明显增多,且TG和ROS水平显著升高,而C_(60)处理使得细胞脂肪蓄积减少,TG和ROS水平均下降,尤其是对氧化应激反应较为敏感的Huh6细胞,C_(60)的改善效果更佳。总之,C_(60)能有效改善肝细胞脂肪变性的程度,并缓解氧化应激所造成的细胞损伤。This study was to investigate the effect of water-soluble fullerene C_(60)on steatotic hepatocytes in vitro.Huh6 and WRL68 cell lines were induced by free fatty acid to establish steatotic hepatocytes models in vitro,and C_(60)was added to intervene the cells.Nile red staining was used to detect lipid accumulation.TG(triglyceride),ROS(reactive oxygen species),SOD(superoxide dismutase),GSH(glutathione)and ATP(adenosine triphosphate)were measured.The results showed that the accumulation of lipid droplets as well as the levels of TG and ROS were significantly increased in the cells induced by free fatty acids,but decreased in the cells treated with C_(60).In particular,for Huh6 cells which were sensitive to oxidative stress,C_(60)had better effect.In conclusion,C_(60)can effectively reverse the degree of steatosis and protect cell from injury induced by oxidative stress.

关 键 词：NAFLD 富勒烯C_(60) 游离脂肪酸 肝细胞脂肪变性 

分 类 号：R575.5[医药卫生—消化系统]