苜蓿银纹夜蛾核型多角体病毒对草地贪夜蛾幼虫的杀虫活性及生防效果  

Insecticidal activity and bio-control effect of Autographa californica multiple nucleopolyhedrovirus on larvae of Spodoptera frugiperda

作　　者：吕亮[1] 陈娇 郭志敏 吕为 陶佩文 吕惠红 潘哲群 常向前[1] 万鹏[1] 张舒[1] Lü Liang;CHEN Jiao;GUO Zhiming;Lü Wei;TAO Peiwen;Lü Huihong;PAN Zhequn;CHANG Xiangqian;WAN Peng;ZHANG Shu(Key Laboratory of Integrated Pest Management on Crops in Central China,Ministry of Agriculture and Rural Affairs/Institute for Plant Protection and Soil Fertilizer,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Wuhan Unioasis Biological Technology Co.Ltd,Wuhan 430072,China;College of Plant Science and Technology,Huzhong Agricultural University,Wuhan 430070,China)

机构地区：[1]农业农村部华中作物有害生物综合治理重点实验室/湖北省农业科学院植保土肥研究所,武汉430064 [2]武汉武大绿洲生物技术有限公司,武汉430072 [3]华中农业大学植物科学技术学院,武汉430070

出　　处：《华中农业大学学报》2021年第5期46-53,共8页Journal of Huazhong Agricultural University

基　　金：湖北省重点研发计划项目(2020BBA051);湖北省农业科技创新行动项目(NYKJ2019011)。

摘　　要：为明确苜蓿银纹夜蛾核型多角体病毒(Autographa californica multiple nucleopolyhedrovirus,AcMNPV)对草地贪夜蛾幼虫的防治效果,采用室内生物活性测定、田间药效试验等方法,研究和分析AcMNPV对草地贪夜蛾幼虫的杀虫活性和生防效果,结果显示,AcMNPV对草地贪夜蛾2龄幼虫的LC_(50)=2.9×10^(7)PIB/mL;在田间,AcMNPV+Bt复配制剂1千万苜核·苏云菌悬浮剂(1500 mL/hm^(2))药后第10天的平均虫口防效为68.99%,而药后第15天的平均防效亦有66.87%,均对草地贪夜蛾幼虫表现出较好的防治效果。应用DNAMAN 6.0软件对试验后死虫样进行DNA同源比对鉴定,结果显示polh、lef-8和lef-9基因序列同源性均达到100%。以上结果表明AcMNPV对草地贪夜蛾幼虫有较好的控制作用。建议在草地贪夜蛾低龄幼虫发生高峰期施用1千万苜核·苏云菌悬浮剂,最好选择在晴天16:00-17:00使用,避开高温和光照等不利环境条件的影响,以使病毒制剂能更好地发挥作用,提高其防治效果。Fall armyworm,Spodoptera frugiperda,is one of the most important pests on maize,which invaded into China newly.In order to determine the control effect of Autographa californica multiple nucleopolyhedrovirus(AcMNPV)on larvae of S.frugiperda,the insecticidal activity and biocontrol effect of AcMNPV on S.frugiperda were studied and analyzed by the methods of bioassay and field efficacy test.The results showed that the median lethal concentration(LC_(50))of AcMNPV acting on the 2 nd instar larvae of S.frugiperda was 2.9×10^(7)PIB/mL.The average control efficacy of 10^(7)PIB/mL AcMNPV+Bt Suspension(1500 mL/hm^(2))on S.frugiperda was 68.99%on the 10 th day and 66.87%on the 15 th day after administration.Finally,DNAMAN 6.0 software was used to identify the DNA homology of the dead insects,and the results showed that the polh,lef-8 and lef-9 gene sequences sequences of the dead insect and the S.frugiperda were 100%identical.All the above results could further verify that AcMNPV may play a key role on controlling S.frugiperda.It is suggested that 10^(7)PIB/mL AcMNPV+Bt Suspension(1500 mL/hm^(2))should be applied at the peak of the occurrence of the young larvae of S.frugiperda,and 4:00-5:00 pm on sunny day to avoid the influence of high temperature and light,so that the virus preparation can play a better role and improve its control effect on S.frugiperda.

关 键 词：苜蓿银纹夜蛾核型多角体病毒 草地贪夜蛾 鳞翅目害虫 幼虫 生物农药 苜核·苏云菌悬浮剂 杀虫活性 绿色防控 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]