HILPDA在胃癌中表达及与肿瘤侵袭迁移的关系  

Expression of HILPDA in gastric cancer and its relationship with tumor invasion and migration

作　　者：赵雪峰[1] 谭明[1] 赵一杰 檀碧波[1] ZHAO Xuefeng;TAN Ming;ZHAO Yijie(Third Department of Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050019,China)

机构地区：[1]河北医科大学第四医院外三科,河北省石家庄市050019

出　　处：《临床合理用药杂志》2021年第24期11-14,18,共5页Chinese Journal of Clinical Rational Drug Use

基　　金：河北省科技厅健康医疗与生物医药专项(182777124D)。

摘　　要：目的探讨胃癌HILPDA表达的临床意义,进一步分析HILPDA与肿瘤侵袭迁移的关系及机制。方法应用生物信息学方法在TCGA数据库检索相应数据,对胃癌及癌旁组织中HILPDA基因的表达差异及预后进行分析。取35例胃癌手术标本对生物信息学结果进行验证。应用GSEA富集分析法对HILPDA的作用机制进行探讨。结果 (1)生物信息学结果显示,HILPDA在胃癌组织中HILPDA表达高于癌旁组织;HILPDA高表达是胃癌患者预后差的标志(均P<0.05);对临床组织验证的结果与生物信息学结果符合。(2)GSEA富集分析显示,HILPDA高表达组中存在乏氧、DNA修复、凋亡、P53等通路的基因富集,通路中VEGFA、PCNA、CDK2、HMGB2等基因与HILPDA存在共表达。(3)HILPDA表达水平与胃癌组织的患者临床分期和淋巴结转移情况有关(均P<0.05);Spearman相关分析显示,HILPDA与VEGFA、PCNA、CDK2的表达均存在正相关(r=0.396,P=0.019;r=0.462,P=0.005;r=0.548,P<0.001)。结论胃癌组织中HILPDA表达较癌旁组织增高,并且与患者预后有关。HILPDA可能通过调控肿瘤侵袭相关基因促进胃癌的进展及转移。Objective To explore the clinical value of HILPDA expression in gastric cancer,and to further analyze the relationship and mechanism of HILPDA with tumor invasion and migration.Methods Bioinformatics analysis was applied to download relevant data in the TCGA database,to analyze the difference of HILPDA gene expression and prognosis in gastric cancer and paracancer tissues.35 cases of gastric cancer surgery specimens were collected to verify the bioinformatics results.The GSEA enrichment analysis method was used to explore the mechanism of HILPDA.Results (1)Bioinformatics results showed that the expression of HILPDA in gastric cancer tissues was higher than that in paracancer tissues;over-expression of HILPDA was a sign of poor prognosis in gastric cancer patients(all P<0.05);the results of clinical tissue verification were consistent with bioinformatics results.(2)GSEA enrichment analysis showed that there was gene enrichment in the pathways of hypoxia,DNA repair,apoptosis,and P53 in the HILPDA high expression group,and genes such as VEGFA,PCNA,CDK2,HMGB2 and HILPDA were co-expressed in the pathway.(3)The expression level of HILPDA was related to the clinical stage and lymph node metastasis of patients with gastric cancer tissues(all P<0.05);spearman correlation analysis shows that HILPDA was positively correlated with VEGFA,PCNA,and CDK2(r=0.396,P=0.019;r=0.462,P=0.005;r=0.548,P<0.001).Conclusion The expression of HILPDA in gastric cancer is higher than that in paracancer tissues,and it is related to the prognosis of patients.HILPDA may promote the progression and metastasis of gastric cancer by regulating genes related to tumor invasion.

关 键 词：胃癌 HILPDA基因 侵袭 迁移 生物信息学分析 

分 类 号：R735.2[医药卫生—肿瘤]