LAMA3在胰腺癌中的预后价值及机制的生物信息学分析  

Bioinformatics analysis of the prognostic value and mechanism of LAMA3 in pancreatic cancer

作　　者：王维杰[1] 赵森峰 曹家辉 李冰洁[3] WANG Wei-jie;ZHAO Sen-feng;CAO Jia-hui;LI Bing-jie(Department of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Medical Science Academy,Zhengzhou University,Zhengzhou 450052,China;Department of Oncology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院肝胆胰外五科,河南郑州450052 [2]郑州大学医学科学院,河南郑州450052 [3]郑州大学第一附属医院肿瘤科,河南郑州450052

出　　处：《肝胆胰外科杂志》2021年第9期533-538,共6页Journal of Hepatopancreatobiliary Surgery

基　　金：国家自然科学基金项目(81900558);河南省医学科技攻关省部共建青年项目(SBGJ202003035)。

摘　　要：目的通过生物信息学分析LAMA3的表达对胰腺癌预后的诊断价值及其与肿瘤临床特征的相关性。方法从TCGA和GEO数据库分别下载胰腺癌转录组及临床数据,R软件用于数据处理和分析。首先明确LAMA3的差异表达,继而Kaplan-Meier分析LAMA3的表达与胰腺癌总生存时间的关系,进而采用Cox回归分析LAMA3的表达与临床病理特征的相关性。最后利用GSEA预测分析LAMA3在胰腺癌中可能的分子机制。结果差异表达分析提示LAMA3在胰腺癌中显著高表达(P<0.05);生存分析提示LAMA3高表达的患者总体生存期明显短于低表达患者;单因素及多因素Cox回归分析提示LAMA3可以作为胰腺癌的独立预后因子。GSEA通路富集分析表明:p53信号通路、细胞周期、DNA复制、剪接体、蛋白酶体及氧化磷酸化在LAMA3高表达表型中富集;细胞因子-细胞因子受体相互作用和细胞黏附分子在LAMA3低表达表型中富集。结论LAMA3高表达是胰腺癌一个独立的不良预后因子,促进了胰腺癌的增殖、侵袭和转移。Objective To analyze the prognostic value of Laminin subunit alpha 3(LAMA3)expression for pancreatic cancer and its correlation with clinical characteristics of tumor with bioinformatics analysis.Methods The pancreatic cancer transcription profile data and clinical data were downloaded from the Cancer Genome Alas(TCGA)and Gene Expression Omnibus(GEO)database respectively.R Project was used for data processing and analysis.Firstly,analysis on the difference expression of LAMA3 was conducted.Then Kaplan-Meier analysis was employed to analyze the relation between LAMA3 expression and total survival(OS),and Cox regression was used to analyze the clinical pathological characteristics.Finally,gene set enrichment analysis(GSEA)was performed to investigate the possible molecular mechanism.Results The differential expression analysis suggested that LAMA3 had significantly higher expression(P<0.05)in pancreatic cancer.The survival analysis suggested that the overall survival of patients with high expression of LAMA3 was significantly shorter than that of patients with low expression.Furthermore,the Cox regression analysis suggested that LAMA3 was an independent factor in predicting the survival outcomes for pancreatic cancer patients.GSEA pathway enrichment analysis showed that p53 signaling pathways,cell cycles,DNA replication,spliceosome,proteasome and oxidation phosphorylation were enriched in LAMA3 high expression profiles,while cytokine-cytokine receptor interactions and cell adhesion molecules were enriched in LAMA3 low expression profiles.Conclusion LAMA3 is an independent prognostic factor for pancreatic cancer,which may contribute to the proliferation,invasion and metastasis of pancreatic cancer.

关 键 词：胰腺癌 LAMA3 生物信息学分析 肿瘤预后 TCGA数据库 GEO数据库 基因集富集分析 KEGG数据库 

分 类 号：R735.9[医药卫生—肿瘤]