NSCLC组织中8-OHdG、p53的表达与患者临床病理特征及预后的关系  

Expression of 8-OHdG and p53 in NSCLC tissues and its relationship with clinicopathological characteristics and prognosis of patients

作　　者：贠宇辉 景鑫 杨恩德 YUN Yuhui;JING Xin;YANG Ende(Depatement of Thoracic Surgery,Tangdu Hospital/Second Affiliated Hospital of PLA Air Force Military Medical University,Xi′an,Shaanxi 710038,China)

机构地区：[1]空军军医大学唐都医院/空军军医大学第二附属医院胸腔外科,陕西西安710038

出　　处：《检验医学与临床》2021年第18期2695-2699,共5页Laboratory Medicine and Clinic

摘　　要：目的探讨非小细胞肺癌(NSCLC)组织中8-羟基脱氧鸟苷(8-OHdG)、人抑癌基因p53的表达与临床病理特征及预后的关系。方法应用免疫组织化学方法检测203例NSCLC患者组织中8-OHdG和p53的表达情况,并分析其与患者临床病理特征的相关性;绘制Kaplan-Meier生存曲线,采用Log-rank检验比较不同临床病理特征NSCLC患者的总生存率;采用单因素和多因素Cox比例风险回归模型分析影响NSCLC患者生存期的危险因素。结果83.3%(169/203)的NSCLC组织呈8-OHdG阳性表达,且8-OHdG阴性表达与男性、鳞癌、T分期高、N1期、吸烟有关(P<0.05)。p53异常表达与男性、鳞癌、吸烟及Ki-67阳性表达有关(P<0.05)。单因素Cox比例风险回归模型分析显示,年龄≥65岁、男性、吸烟、T分期高、N1分期、8-OHdG阴性表达、p53异常表达及Ki-67阳性表达是影响NSCLC患者生存期的危险因素(P<0.05);多因素Cox比例风险回归模型分析显示,8-OHdG阴性表达和p53异常表达并不是影响NSCLC患者生存期的独立危险因素(HR=1.486、1.211,P>0.05)。结论8-OHdG阴性表达和p53异常表达与吸烟和较差的预后有关,但二者不是影响NSCLC患者生存期的独立危险因素。Objective To investigate the expression of 8-Hydroxydeoxyguanosine(8-OHdG)and human tumor suppressor gene p53 in non-small cell lung cancer(NSCLC)and its relationship with smoking and clinicopathological features and prognosis.Methods Immunohistochemical method was used to detect the expression of 8-OhdG and p53 in 203 patients with NSCLC,and analyze their correlation with the clinical pathological characteristics of the patients.Draw a Kaplan-Meier survival curve,and use Log-rank test to compare the overall survival rate of NSCLC patients with different clinicopathological characteristics.Univariate and multivariate Cox proportional hazards regression models were used to analyze the risk factors affecting the survival of NSCLC patients.Results 83.3%(169/203)of NSCLC tissues showed positive expression of 8-OHdG,and the negative expression of 8-OHdG was associated with male,squamous cell carcinoma,high T stage,N1 stage and smoking(P<0.05).Abnormal expression of p53 was associated with male,squamous cell carcinoma,smoking and positive expression of Ki-67(P<0.05).Univariate Cox proportional hazard regression model analysis showed that age≥65 years old,male,smoking,high T stage,N1 stage,negative expression of 8-OHdG,abnormal expression of p53,and positive expression of Ki-67 are risk factors that affect the survival of NSCLC patients(P<0.05).Multivariate Cox proportional hazard regression model analysis showed that the negative expression of 8-OHdG and abnormal expression of p53 were not independent risk factors affecting the survival of NSCLC patients(HR=1.486,1.211,P>0.05).Conclusion The negative expression of 8-OHdG and abnormal expression of p53 are related to smoking and poor prognostic,but but they are not independent risk factors affecting overall survival in NSCLC patients.

关 键 词：非小细胞肺癌 8-羟基脱氧鸟苷 人抑癌基因p53 预后 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤] R446.9[医药卫生—临床医学]