可视化LAMP检测阿胶及其保健食品中马源性成分  

Determination of Horse-Derived Components in Asini corii Col a and Health Foods by Visualization Loop-Mediated Isothermal Amplification

作　　者：高玉梅[1] 柳毅[2] 李洪[3] 尹斯雅[2] GAO Yumei;LIU Yi;LI Hong;YIN Siya(Medical College,Hebei University of Engineering,Handan 056002;Baoding Product Quality Supervision and Inspection Institute,Baoding 071051;Hebei Chemical&Pharmaceutical College,Shijiazhuang 050026)

机构地区：[1]河北工程大学医学院,邯郸056002 [2]保定市产品质量监督检验所,保定071051 [3]河北化工医药职业技术学院,石家庄050026

出　　处：《食品工业》2021年第8期282-285,共4页The Food Industry

基　　金：河北省自然科学基金项目(C2019204342);河北省重点研究开发项目(18275501D)。

摘　　要：建立阿胶及其保健食品中马源性成分环介导等温扩增检测方法,对市售阿胶及其保健食品进行马源性成分检测分析。特异性引物选用马的线粒体ATPase 6基因,优化可视化LAMP反应条件,验证引物的特异性和灵敏度。可视化LAMP对阿胶及其保健食品中马源性成分的灵敏度为0.0001 ng/μL,是实时荧光PCR法的100倍。该检测方法操作简便、专属性强、灵敏度高,适合于阿胶及其保健食品生产企业和基层市场监督管理部门。A method of loop-mediated isothermal amplification for detection of horse derived components in Asini corii Colla and health foods was developed.Specific primers were designed for the mitochondrial ATPase 6 gene of the horse,and the LAMP reaction conditions were optimized to verify the specificity of the primers.The sensitivity of visual LAMP to equine compounds in Asini corii Colla and health foods was 0.0001 ng/μL,which was 100 times that of real-time PCR.The method was simple,specific and sensitive,and was suitable for Asini Corii Colla and health foods enterprises and grass roots market supervision and management departments.

关 键 词：环介导等温扩增技术 可视化检测 阿胶 马源性成分 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]