GATA3和bcl-11b在外周T细胞淋巴瘤中的表达及临床意义  

Expression of GATA3 and bcl-11b in peripheral T-cell lymphoma and their clinical significance

作　　者：王雅婧 张红玲 李静[3] 步鹏[3] 张飞[1] 郗彦凤[3] Wang Yajing;Zhang Hongling;Li Jing;Bu Peng;Zhang Fei;Xi Yanfeng(School of Basic Medicine,Shanxi Medical University,Taiyuan 030000,China;Department of Pathology,Second Medical College of Shanxi Medical University,Taiyuan 030000,China;Department of Pathology,Shanxi Cancer Hospital,Taiyuan 030000,China)

机构地区：[1]山西医科大学基础医学院,太原030000 [2]山西医科大学第二临床医学院病理科,太原030000 [3]山西省肿瘤医院病理科,太原030000

出　　处：《中华病理学杂志》2021年第8期904-909,共6页Chinese Journal of Pathology

摘　　要：目的探究GATA3和bcl-11b在外周T细胞淋巴瘤(peripheral T-cell lymphoma,PTCL)中的表达情况及其与临床病理特征之间的关系。方法利用Oncomine数据库和GEO数据库分析GATA3和bcl-11b mRNA在PTCL中的表达水平;收集山西省肿瘤医院2010年1月至2020年6月间诊断为PTCL的127例患者病例资料及石蜡标本,制作组织芯片,采用免疫组织化学染色(IHC)方法检测GATA3和bcl-11b蛋白在127例PTCL患者组织标本及40例反应性增生淋巴结中的表达情况。结果Oncomine数据库和GEO数据库中的数据显示,GATA3和bcl-11b mRNA在PTCL组织中表达低于正常组织(P<0.05)。IHC结果显示,GATA3在PTCL和淋巴结反应增生性淋巴组织表达率分别为60.6%(77/127)和85.0%(34/40;P<0.05),bcl-11b在PTCL和淋巴结反应增生性淋巴组织表达率分别为55.1%(70/127)和75.0%(30/40;P<0.05)。GATA3的表达与PTCL患者病理分型有关,与患者Ann Arbor分期呈负相关;bcl-11b的表达与患者国际预后指数评分呈负相关(P<0.05),二者表达与患者年龄、性别、乳酸脱氢酶水平、B症状等临床病理特征均无关(P>0.05);Spearman相关分析显示,PTCL组织中GATA3蛋白与bcl-11b蛋白的表达呈正相关。结论GATA3和bcl-11b与PTCL的预后密切相关,可能是PTCL发生发展的重要参与因子。Objective To study the expression of GATA3 and bcl-11b in peripheral T-cell lymphoma(PTCL)and their correlation with clinicopathological features.Methods The Oncomine and GEO databases were used for analyzing the expression levels of GATA3 and bcl-11b mRNA in PTCL.Immunohistochemistry was used to detect the expression of GATA3 and bcl-11b proteins in 127 cases of PTCL diagnosed at Shanxi Provincial Cancer Hospital from January 2010 to June 2020,as well as 40 cases of lymph node with reactive hyperplasia.Results The data in Oncomine and GEO databases showed that the expression of GATA3 and bcl-11b mRNA in PTCL was lower than that in normal tissues(P<0.05).Immunohistochemistry showed that the positive rates of GATA3 in PTCL and lymph nodes with reactive hyperplasia were 60.6%(77/127)and 85.0%(34/40,P<0.05),respectively.The expression rates of bcl-11b in PTCL and lymph nodes with reactive hyperplasia were 55.1%(70/127)and 75.0%(30/40,P<0.05),respectively.The expression of GATA3 was related to the pathological classification of the patients with PTCL,and was inversely related to the Ann Arbor stage of the patient,while the expression of bcl-11b was inversely correlated with the IPI score of the patient(P<0.05).The expression of GATA3 and bcl-11b was related to the patients′age,gender,LDH level,and B symptoms.Other clinicopathological characteristics were irrelevant.Spearman correlation analysis shows that the expression of GATA3 protein was associated with that of bcl-11b protein in PTCL.Conclusion GATA3 and bcl-11b are closely related to the prognosis of PTCL,and may be important factors involved in the occurrence and development of PTCL.

关 键 词：淋巴瘤 T细胞 外周 数据库 预后 

分 类 号：R733.1[医药卫生—肿瘤]