Targeted Micellar Phthalocyanine for Lymph Node Metastasis Homing and Photothermal Therapy in an Orthotopic Colorectal Tumor Model  

作　　者：Hai‑Yi Feng Yihang Yuan Yunpeng Zhang Hai‑Jun Liu Xiao Dong Si‑Cong Yang Xue‑Liang Liu Xing Lai Mao‑Hua Zhu Jue Wang Qin Lu Quanjun Lin Hong‑Zhuan Chen Jonathan F.Lovell Peng Sun Chao Fang 

机构地区：[1]Hongqiao International Institute of Medicine,Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes,Department of Pharmacology and Chemical Biology,Shanghai Jiao Tong University School of Medicine(SJTU-SM),Shanghai 200025,People’s Republic of China [2]Department of General Surgery,Tongren Hospital,SJTU-SM,Shanghai 200336,People’s Republic of China [3]Institute of Interdisciplinary Integrative Biomedical Research,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,People’s Republic of China [4]Department of Biomedical Engineering,University at Buffalo,State University of New York,Buffalo,NY 14260,USA

出　　处：《Nano-Micro Letters》2021年第11期1-17,共17页纳微快报（英文版）

基　　金：Hai-Yi Feng and Yihang Yuan contributed equally to this work.We thank Prof.Gang Zheng(University of Toronto)for valuable discussion.We also thank the Core Facility of Basic Medical Sciences(SJTU-SM)for frozen section making and scanning;This work was supported by National Natural Science Foundation of China(81572998,81773274,82073379);Shanghai Municipal Science and Technology Commission(20ZR1451700,16520710700);Shanghai Collaborative Innovation Center for Translational Medicine(TM201731).

摘　　要：Tumor lymph node(LN)metastasis seriously affects the treatment prognosis.Studies have shown that nanoparticles with size of sub-50 nm can directly penetrate into LN metastases after intravenous administration.Here,we speculate through introducing targeting capacity,the nanoparticle accumulation in LN metastases would be further enhanced for improved local treatment such as photothermal therapy.Trastuzumabtargeted micelles(<50 nm)were formulated using a unique surfactantstripping approach that yielded concentrated phthalocyanines with strong near-infrared absorption.Targeted micellar phthalocyanine(T-MP)was an effective photothermal transducer and ablated HT-29 cells in vitro.A HER2-expressing colorectal cancer cell line(HT-29)was used to establish an orthotopic mouse model that developed metastatic disease in mesenteric sentinel LN.T-MP accumulated more in the LN metastases compared to the micelles conjugated with control IgG.Following surgical resection of the primary tumor,minimally invasive photothermal treatment of the metastatic LN with T-MP,but not the control micelles,extended mouse survival.Our findings demonstrate for the first time that targeted small-sized nanoparticles have potential to enable superior paradigms for dealing with LN metastases.

关 键 词：Lymph node metastasis Photothermal therapy TRASTUZUMAB PHTHALOCYANINE Micelles 

分 类 号：R735.34[医药卫生—肿瘤]