基于PTEN/AKT信号通路分析miR-128对宫颈癌SiHa细胞增殖、侵袭作用的影响  

Effects of miR-128 on Proliferation and Invasion of SiHa Cells Based on PTEN/Akt Signaling Pathway

作　　者：庞俊芳 梁宗志 李斌 黄亚鹏 张清伟 PANG Junfang;LIANG Zongzhi;LI Bin(Henan Provincial Hospital,Zhengzhou,450019)

机构地区：[1]河南省省立医院,450019

出　　处：《实用癌症杂志》2021年第8期1224-1227,共4页The Practical Journal of Cancer

基　　金：河南省医学科技攻关计划项目(编号:2018021026);2020年度河南省重点研发与推广专项【科技攻关】类项目(编号:2020-LYZKYYB047)。

摘　　要：目的在PTEN/AKT信号通路的基础上分析miR-128对宫颈癌SiHa细胞增殖、侵袭作用的影响。方法将SiHa细胞进行传代培养,用miR-128-mimic和miR-128-inhibitor将SiHa细胞进行转染,分别设为观察组和阴性对照组,以及未做处理的SiHa细胞作为空白对照组。应用RT-PCR技术和采用Western Blot免疫印迹法,检测观察组和阴性组mRNA和蛋白表达水平,MTT法和Transwell小室法检测三组SiHa细胞的增殖、侵袭能力。结果观察组miR-128表达水平高于阴性组,PTEN mRNA、蛋白表达水平均低于阴性组,p-AKT蛋白表达水平高于阴性组,组间差异具有统计学意义(P<0.05);观察组和阴性组SiHa细胞的增殖、侵袭能力均低于空白对照组,并且观察组的增殖、侵袭能力更低,组间比较具有统计学差异(P<0.05)。miR-128对PTEN mRNA以及蛋白的表达起到抑制作用,激活PTEN/AKT信号通路,升高AKT的磷酸化水平,过表达miR-128对宫颈癌细胞的进一步发生发展起到抑制作用。结论miR-128和PTEN的过表达均能抑制宫颈癌SiHa细胞的增殖、侵袭能力,但miR-128抑制PTEN的表达,miR-128发挥抑癌作用可能与PTEN/AKT信号通路无关,有待于进一步研究。Objective To investigate the effect of miR-128 on the proliferation and invasion of SiHa cells based on PTEN/Akt signaling pathway.Methods SiHa cells were subcultured and transfected with miR-128 mimic and miR-128 inhibitor,respectively.SiHa cells were set as observation group and negative control group,and untreated SiHa cells were taken as blank control group.RT-PCR and western blot were used to detect the mRNA and protein expression levels of SiHa cells in the observation group and negative group.MTT method and Transwell chamber method were used to detect the proliferation and invasion ability of SiHa cells in the 3 groups.Results The expression level of miR-128 in observation group was higher than that in negative group The expression levels of mRNA and protein were lower in the observation group than in the negative group,and the expression level of p-Akt protein was higher than that in the negative group(P<0.05);the proliferation and invasion ability of SiHa cells in the observation group and the negative group were lower than those in the blank control group,and the proliferation and invasion ability of the observation group were lower than those of the blank control group(P<0.05).MiR-128 can inhibit the expression of PTEN mRNA and protein,activate PTEN/Akt signaling pathway and increase the phosphorylation level of Akt.Overexpression of miR-128 can inhibit the further development of cervical cancer cells.Conclusion Overexpression of miR-128 and PTEN can inhibit the proliferation and invasion of SiHa cells,but miR-128 can inhibit the expression of PTEN.The inhibitory effect of miR-128 may not be related to PTEN/Akt signaling pathway,which needs further study.

关 键 词：PTEN miR-128 宫颈癌 增殖 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]