叶酸缺乏导致正常喉黏膜P15基因CpG岛甲基化与喉癌发生发展的相关性  

Effect of Folate Deficiencythe on Aberrant Methylation of P15 CPG Island in Normal Laryngeal Mucosa on Laryngeal Carcinoma

作　　者：赵鹏 曾萍 彭德新[1] 彭丽香[1] ZHAO Peng;ZENG Ping;PENG Dexing(Jiangxi Cancer Hospital,Nanchang,330029)

机构地区：[1]江西省肿瘤医院,330029 [2]江西省儿童医院,330000

出　　处：《实用癌症杂志》2021年第8期1236-1238,共3页The Practical Journal of Cancer

基　　金：江西省卫生计生委科技计划(编号:20185365);江西省卫生计生委科技计划(编号:20182337)。

摘　　要：目的通过甲基化芯片(DNA Immunoprecipitation chip assay)对叶酸缺乏导致人正常喉黏膜细胞的DNA甲基化状态变化情况进行筛查,探讨叶酸缺乏引起抑癌基因P15 CpG岛甲基化异常与喉癌的关系。方法通过甲基化芯片对低叶酸浓度培养的喉黏膜细胞中基因启动子区CpG岛的甲基化状态进行检测,筛查出的相应位点在喉癌组织样本进行验证。结果通过甲基化芯片从低叶酸组筛选到了11个基因启动子区CpG岛发生了超甲基化的改变。与正常喉黏膜组织对比,癌组织中P15基因甲基化更高,但P15基因甲基化与喉癌临床病理特征无关(P>0.05)。结论叶酸缺乏导致人正常喉黏膜上皮细胞DNA甲基化状态的改变可能与喉癌的发生相关。Objective To investigate the methylation patterns changes induced by folate deficiency in normal human laryngeal mucosa cell line and the effects of methylation on P15 expression during the development of laryngeal squamous cell carcinoma(LSCC).Methods Methylated DNA immunoprecipitation chip assay was used in folate deficiency in normal human laryngeal mucosa cell line,and verified the results of the assay in the screened corresponding sites and tissue samples of laryngeal cancer patients.Results DNA immunoprecipitation chip assay was used in folate deficiency in normal human laryngeal mucosa cell line in this study.Compared with normal human laryngeal mucosa tissue,Methylation of P15 in cancer tissue is higher,but methylation of P15 was not related with clinicopathological features of laryngocarcinoma(P>0.05).Conclusion Folate deficiency could induce aberrant genes methylation and expression in LSCC.Some related genes play roles in tumorigenesis of laryngocarcinoma.

关 键 词：甲基化 CPG岛 喉癌 叶酸 

分 类 号：R739.65[医药卫生—肿瘤]