抑制SLP-2对宫颈癌细胞迁移及侵袭能力的影响  

Inhibition of stomatin-like protein 2 on migration and invasion of cervical cancer cells

作　　者：赵晶蕾 韦广月 刘艳洁 ZHAO Jinglei;WEI Guangyue;LIU Yanjie(Department of Pathology and Pathophysiology,School of Basic Medicine College,Guizhou Medical University,Guiyang 561000,China)

机构地区：[1]贵州医科大学基础医学院病理与病理生理学系,贵州贵阳561000

出　　处：《西部医学》2021年第9期1316-1319,1325,共5页Medical Journal of West China

基　　金：贵州省科技计划项目[黔科合支撑(2019)2791号]。

摘　　要：目的探讨抑制stomatin样蛋白2(SLP-2)对宫颈癌细胞的迁移及侵袭能力的影响。方法将人宫颈腺癌Hela细胞和鳞癌Siha细胞分别分为siRNA阴性对照组(siRNA-CON)和SLP2-siRNA组。采用western blot检测转染后各组细胞SLP-2蛋白表达水平;Transwell小室试验检测细胞侵袭能力;细胞划痕试验检测细胞迁移能力;western blot检测侵袭相关蛋白Rac1、基质金属蛋白酶-2(MMP-2)和MMP-9表达水平。采用独立样本t检验进行统计学分析。结果Western blot检测结果显示,SLP2-siRNA组Hela细胞和Siha细胞中SLP-2蛋白水平均低于siRNA-CON组,差异有统计学意义(P<0.05)。Transwell小室试验结果显示,SLP2-siRNA组Hela细胞和Siha细胞侵袭能力低于siRNA-CON组,差异有统计学意义(P<0.05)。细胞划痕试验结果显示,SLP2-siRNA组Hela细胞和Siha细胞24h和36h迁移能力低于siRNA-CON组,差异有统计学意义(P<0.05)。Western blot检测结果显示,SLP2-siRNA组Hela细胞和Siha细胞中Rac1、MMP-2、MMP-9蛋白表达水平均低于siRNA-CON组,差异有统计学意义(P<0.05)。结论干扰SLP-2表达后宫颈腺癌Hela细胞和鳞癌Siha细胞的侵袭能力和迁移能力明显下降,提示SLP-2在宫颈癌进展中有着重要作用,可能是潜在的转移预测标志物和治疗靶点。Objective To investigate the effect of inhibiting stomatin-like protein 2(SLP-2)on migration and invasion of cervical cancer cells.Methods Human cervical adenocarcinoma Hela cells and squamous cell carcinoma Siha cells were divided into siRNA negative control group(siRNA-CON)and SLP2-siRNA group,respectively.Western blot was used to detect the expression of SLP-2 protein in each group after transfection.Transwell lab test was used to detect the invasive ability of cells.Scratch test was used to detect the migration ability of cells.Western blot was used to detect the expression levels of invasive related proteins Rac1,matrix metalloproteinase-2(MMP-2)and MMP-9.Independent sample t test was used for statistical analysis.Results Western blot results showed that SLP-2 protein levels in Hela cells and Siha cells in SLP2-siRNA group were lower than those in siRNA-CON group,and the difference was statistically significant(P<0.05).Transwell lab test showed that the invasive ability of Hela cells and Siha cells in SLP2-siRNA group was lower than that in siRNA-CON group(P<0.05).The results of cell scratch test showed that the migration ability of Hela cells and Siha cells in SLP2-siRNA group was lower than that in siRNA-CON group at 24h and 36h(P<0.05).Western blot results showed that the expression levels of Rac1,MMP-2 and MMP-9 in Hela cells and Siha cells in SLP2-siRNA group were lower than those in siRNA-CON group(P<0.05).Conclusion The invasive and migratory abilities of Hela cells and Siha cells of cervical adenocarcinoma decreased significantly after the interference of SLP-2 expression,suggesting that SLP-2 plays an important role in the progression of cervical cancer and may be a potential predictor of metastasis and therapeutic target.

关 键 词：stomatin样蛋白2 宫颈癌 HELA细胞 SIHA细胞 细胞侵袭 细胞迁移 

分 类 号：R737.33[医药卫生—肿瘤]