SCNN1B在非小细胞肺癌组织中的表达及其调节肺癌对顺铂的敏感性  

Expression of SCNN1B in non-small cell lung cancer tissues and its regulation for lung cancer sensitivity to cisplatin

作　　者：丁家宝 高鲲[1] 张倩 梁华刚[1] 刘赫 李建 DING Jiabao;GAO Kun;ZHANG Qian;LIANG Huagang;LIU He;LI Jian(Department of Thoracic Surgery,The First Hospital of Qinhuangdao,Qinhuangdao 066000,Hebei,China;Department of Rheumatology and Immunology,The First Hospital of Qinhuangdao,Qinhuangdao 066000,Hebei,China)

机构地区：[1]秦皇岛市第一医院胸外科,河北秦皇岛066000 [2]秦皇岛市第一医院风湿免疫科,河北秦皇岛066000

出　　处：《西部医学》2021年第9期1354-1358,共5页Medical Journal of West China

基　　金：秦皇岛市科学技术研究与发展计划(202004A059)。

摘　　要：目的探讨SCNN1B在非小细胞肺癌(NSCLC)组织中的表达及其调节肺癌对顺铂的敏感性。方法选取本院2017年12月~2019年12月收治的130例NSCLC患者,RT-PCR法检测其肺癌组织中SCNN1B mRNA水平;另设A549细胞组、A549/DDP细胞组、siRNA-SCNN1B组、siRNA-NC组,其中siRNA-SCNN1B组、siRNA-NC组分别转染SCNN1B过表达质粒及空载质粒(阴性对照),RT-PCR法检测各组SCNN1B mRNA相对表达量,验证SCNN1B转染效果;并检测各组半数抑制浓度(IC50)、细胞凋亡、细胞周期,Western blot法检测耐药相关蛋白表达。结果低分化NSCLC患者SCNN1B mRNA低表达率明显高于中高分化患者,TNM分期Ⅳ期患者中SCNN1B mRNA低表达率明显高于Ⅲ期(P<0.05);A549/DDP细胞组SCNN1B mRNA水平显著低于A549细胞组(P<0.05);与A549/DDP细胞组比较,siRNA-SCNN1B组SCNN1B mRNA水平明显上升(P<0.05);siRNA-SCNN1B组IC50明显低于siRNA-NC组,逆转系数下降(P<0.05);与A549细胞组比较,A549/DDP细胞组细胞凋亡率明显下降,细胞周期G0/G1期比例明显上升(P<0.05),siRNA-SCNN1B组细胞凋亡率则较A549/DDP细胞组明显上升,细胞周期G0/G1期比例较A549/DDP细胞组明显下降(P<0.05);与A549细胞组比较,A549/DDP细胞组Bcl-2、生存素、周期蛋白D1(CyclinD1)、表皮生长因子受体(EGFR)、多药耐药相关蛋白-1(MRP1)、肺耐药相关蛋白(LRP)水平明显上升(P<0.05);siRNA-SCNN1B组Bcl-2、生存素、Cyclin D1、EGFR、MPR1、LRP水平则较A549/DDP细胞组明显下降(P<0.05)。结论SCNN1B与NSCLC患者肺癌组织分化程度、肿瘤分期密切相关,靶向上调SCNN1B可下调Bcl-2、生存素、Cyclin D1、EGFR、MPR1、LRP等蛋白表达。Objective To study the expression of SCNN1 B in non-small cell lung cancer(NSCLC) tissues and its regulation for lung cancer sensitivity to cisplatin. Methods The pathological specimens of 130 NSCLC patients who were diagnosed and treated in the hospital from December 2017 to December 2019 were enrolled as the research objects. The level of SCNN1B mRNA in lung cancer tissues of NSCLC patients was detected by RT-PCR. A549 and A549/DDP cell lines were collected, and A549 cell group, A549/DDP cell group, siRNA-SCNN1 B group and siRNA-NC group were set up. The siRNA-SCNN1 B group and siRNA-NC group were transfected with plasmid of SCNN1 B overexpression and empty plasmid(negative control). The relative expression levels of various SCNN1B mRNA were detected by RT-PCR to verify the transfection effect of SCNN1 B. The half inhibitory concentration(IC50), apoptosis and cell cycle in each group were detected. The expression of resistance-related proteins was detected by Western blot. Results The low expression rate of SCNN1 B mRNA in patients with poorly differentiated NSCLC was significantly higher than that with moderately and highly differentiated NSCLC. The low expression rate of SCNN1 B mRNA in patients with TNM staging at stage Ⅳ was significantly higher than that at stage Ⅲ(P<0.05). The level of SCNN1B mRNA in A549/DDP cell group were significantly lower than that in A549 cell group(P<0.05). Compared with A549/DDP cell group, level of SCNN1B mRNA in siRNA-SCNN1 B group was significantly increased(P<0.05). IC50 in siRNA-SCNN1 B group was significantly lower than that in siRNA-NC group, and the reversal coefficient was decreased by(221.69±26.27)%(P<0.05). Compared with A549 cell group, apoptosis rate in A549/DDP cell group was significantly decreased, while proportion of cells at G0/G1 phase was significantly increased(P<0.05). Compared with the A549/DDP cell group, apoptosis rate in siRNA-SCNN1 B group was significantly increased, while proportion of cells at G0/G1 phase was significantly decreased(P<0.05). Com

关 键 词：非小细胞肺癌 SCNN1B 顺铂 敏感性 

分 类 号：R734.2[医药卫生—肿瘤]