非小细胞肺癌组织中circ_0006692的表达及其对肺癌细胞增殖转移的调控机制  

Expression of circ_0006692 in Non-small Cell Lung Cancer and Its Regulatory Mechanism on Proliferation and Metastasis of Lung Cancer Cells

作　　者：陈增 廖锦容 邹长棪[1] 苏颖[1,2] 林可焴[1] 金善丰[3] 郑倩兰 林贤东[1,2] CHEN Zeng;LIAO Jinrong;ZOU Changyan;SU Ying;LIN Keyu;JIN Shanfeng;ZHENG Qianlan;LIN Xiandong(Laboratory of Radiation Oncology and Radiobiology,Fujian Cancer Hospital and Fujian Medical University Cancer Hospital,Fuzhou 350014,China;Fujian Provincial Key Laboratory of Translational Cancer Medicine,Fuzhou 350014,China;Department of Pathology,Fujian Cancer Hospital and Fujian Medical University Cancer Hospital,Fuzhou 350014,China)

机构地区：[1]福建医科大学附属福建省肿瘤医院放射生物学及肿瘤放射治疗研究室,福州350014 [2]福建省科技厅转化医学重点实验室,福州350014 [3]福建医科大学附属福建省肿瘤医院病理科,福州350014

出　　处：《肿瘤防治研究》2021年第9期846-852,共7页Cancer Research on Prevention and Treatment

基　　金：福建省卫健委创新课题(2019-CX-5);福建省自然科学基金(2019J01196,2020J011109)。

摘　　要：目的探讨非小细胞肺癌(NSCLC)中circ_0006692的表达与临床病理特征的关系及机制。方法收集50对NSCLC和癌旁组织,qRT-PCR法检测癌和癌旁组织中circ_0006692的表达,并分析其与临床病理特征之间的关系。构建过表达、敲低的circ_0006692的A549肺癌细胞株,MTS、克隆形成实验、划痕创伤愈合实验和Transwell侵袭实验检测circ_0006692表达变化对细胞增殖、迁移和侵袭能力的影响。qRT-PCR和Western blot检测circ_0006692表达变化对EMT相关基因表达的影响。结果非小细胞肺癌组织中circ_0006692表达水平高于癌旁组织(P<0.05),其表达水平与肿瘤大小、TNM分期及肺膜侵犯密切相关(P<0.05)。circ_0006692敲低抑制细胞增殖、侵袭和转移,过表达可促进细胞增殖、侵袭和转移。qRT-PCR和Western blot结果显示circ_0006692敲低抑制A549肺癌细胞EMT相关基因CDH2和MMP7促进CDH1表达。结论非小细胞肺癌组织中circ_0006692的表达上调与肿瘤大小、TNM分期及肺膜侵犯密切相关;circ_0006692表达可调节A549肺癌细胞增殖、侵袭、转移以及EMT进展。Objective To explore the expression of circ_0006692 in NSCLC and its relation with clinicopathological characteristics and related mechanism.Methods We collected 50 pairs of NSCLC tissues and adjacent tissues.The expression of circ_0006692 was detected by qRT-PCR and its relation with clinicopathological features was analyzed.We constructed lung cancer A549 cell line with circ_0006692 overexpression and knockdown.Cell proliferation,migration and invasion were detected by MTS,colony forming,wound healing and Transwell invasion assays.qRT-PCR and Western blot were used to detect the effect of circ_0006692 expression change on EMT-related gene expression.Results The expression of circ_0006692 in NSCLC was significantly higher than that in adjacent tissues(P<0.05).The expression level of circ_0006692 was closely related to tumor size,TNM stage and pulmonary membrane invasion(P<0.05).circ_0006692 knockdown inhibited cell proliferation,invasion and metastasis,while its overexpression promoted cell proliferation,invasion and metastasis.circ_0006692 knockdown inhibited the expression of EMT-related genes CDH2 and MMP7 in A549 cells and promoted the expression of CDH1.Conclusion The upregulated expression of circ_0006692 in NSCLC is closely related to tumor size,TNM stage and pulmonary membrane invasion.circ_0006692 expression could regulate the proliferation,invasion,metastasis and EMT progression of lung cancer A549 cells.

关 键 词：circ_0006692 非小细胞肺癌 增殖 转移 EMT 

分 类 号：R734.2[医药卫生—肿瘤]