牙龈卟啉单胞菌和伴放线聚集杆菌在江苏汉族牙周炎人群中的分布研究  

Prevalence and distribution of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in Jiangsu Han nationality periodontitis patients

作　　者：杨勰 付子波 曹羡梓 陈佳璐 孙颖[1] YANG Xie;FU Zibo;CAO Xianzi;CHEN Jialu;SUN Ying(Department of Periodontology,The Affiliated Stomatological Hospital of Nanjing Medical University,Jiangsu Province Key Laboratory of Oral Diseases,Jiangsu Province Engineering Research Center of Stomatological Translational Medicine,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院牙周病科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,江苏南京210029

出　　处：《口腔生物医学》2021年第2期100-104,共5页Oral Biomedicine

基　　金：国家自然科学基金(81771075);江苏高校优势学科建设工程资助项目(2018-87)。

摘　　要：目的:从龈下菌斑总菌数、牙龈卟啉单胞菌(P.gingivalis)、伴放线聚集杆菌(A.actinomycetemcomitans)及白细胞毒素(LTX)在江苏汉族牙周炎人群中分布的角度,比较牙周病2018年新分类与1999年分类。方法:纳入73名牙周炎患者和26名牙周健康者,分别按照牙周病2018年新分类与1999年分类对牙周炎人群分组。初诊时记录全口探诊深度(PD)、临床附着丧失(CAL)、牙龈指数(GI)和菌斑指数(PLI),并收集龈下菌斑。采用实时定量PCR检测龈下菌斑中总菌、P.gingivalis和A.actinomycetemcomitans数量,采用PCR检测A.actinomycetemcomitans毒力因子LTX的携带率,比较上述指标在不同的牙周炎分类法间的分布差异。结果:慢性牙周炎和侵袭性牙周炎患者之间,总菌和P.gingivalis数量无显著差异(P>0.05),A.actinomycetemcomitans数量和LTX阳性率存在差异(P<0.05),所有检出的LTX均为低毒型。不同牙周炎分期分级组间,菌量和LTX阳性率存在差异(P<0.05)。PD、CAL、GI与总菌、P.gingivalis、A.actinomycetemcomitans数量间存在相关性(P<0.05)。结论:2018年牙周病新分类能较好地反映江苏汉族牙周炎患者的微生物学特征。Objective:To investigate the prevalence and distribution of total bacteria,Porphyromonas gingivalis(P.gingivalis),Aggregatibacter actinomycetemcomitans(A.actinomycetemcomitans)and leukotoxin(LTX)in Jiangsu Han nationality periodontitits patients categorized by classifications in 2018 and 1999 respectively.Methods:73 patients with periodontitis and 26 periodontal healthy individuals were recruited.Periodontal clinical parameters were recorded,including probing depth(PD),clinical attachment loss(CAL),gingival index(GI)and plaque index(PLI).Levels of total bacteria,P.gingivalis and A.actinomycetemcomitans in subgingival plaque were explored by real-time PCR,and LTX was detected by conventional PCR.Results:There were no differences in the levels of total bacteria and P.gingivalis between chronic and aggressive periodontitis(P>0.05),while differences were found when subjects were grouped by staging and grading(P<0.05).Moreover,differences in the levels of A.actinomycetemcomitans and positive rates of LTX between chronic and aggressive periodontitis patients,between StageⅠ-ⅡandⅢ-Ⅳpatients,and between Grade A and C patients were disclosed(P<0.05).All LTXs detected in this study were subtypes of low virulence.Positive correlations were confirmed between periodontal clinical parameters(PD,CAL and GI)and the levels of total bacteria,P.gingivalis and A.actinomycetemcomitans(P<0.05).Conclusions:Classification for periodontal diseases in 2018 reflected the microbiological characteristics of Jiangsu Han nationality periodontitis patients well.

关 键 词：牙龈卟啉单胞菌 伴放线聚集杆菌 白细胞毒素 牙周炎 分类 

分 类 号：R781.4[医药卫生—口腔医学]