Detection of Epstein-Barr virus infection subtype in patients with multiple sclerosis by indirect immunofluorescence assay  

作　　者：Shan-Chao Zhang Lei Liu Rui-Jin Wang Hou-Zhen Tuo Yan-Jun Guo Li Yi De-Xin Wang Jia-Wei Wang 

机构地区：[1]Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China [2]Department of Neurology,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China

出　　处：《Neuroimmunology and Neuroinflammation》2014年第1期24-28,共5页神经免疫与神经炎症（英文版）

基　　金：The work was supported by Beijing Natural Science Foundation(Nos.7102040,7132060);National Natural Science Foundation of China(Nos.81041020,81271311 and 81241039);Traditional Chinese Medicine,Beijing Technology Development Fund(No.SF-2007-III-22),Beijing Excellent Talent Foundation(No.20071-D0300100062);the high-level technical training project funding of the Beijing health system(2011-3-004);the preferential funding scheme for Beijing city staff to go abroad.Conflict of Interest:No.

摘　　要：Aim:The aim was to investigate the infectious conditions of Epstein-Barr virus(EBV)in patients with multiple sclerosis(MS).Methods:Cerebrospinal fluid(CSF)of 20 patients with MS and 20 with other neurological diseases(OND)were tested with indirect immunofluorescence for anti-EBV capsid antigen(EBV-CA)immunoglobulin G(IgG),IgG affinity for anti-EBV-CA,anti-EBV-CA immunoglobulin M(IgM),anti-EBV early antigen(EBV-EA)IgG and anti-EBV nuclear antigen(EBNA)IgG.According to the pattern of antibodies in CSF,infection rates of acute,chronic,primary,recurrent,and past infections were analyzed in the two groups of patients.Results:There were no significant differences in anti-EBV-CA,anti-EBC-EA,and anti-EBNA antigen IgG in CSF between MS and OND patients(P>0.05).The positive rate of low affinity for anti-EBV-CA IgG in MS patients was significantly higher than that for OND patients(75%vs.40%,P<0.05).Furthermore,significant differences in the positive rate of anti-EBV-CA IgM were found between MS and OND patients(70%vs.25%,P<0.05).Of the MS patients,75%were in an EBV acute infection state compared with 40%of OND patients(P<0.05).Conclusion:Acute infection of EBV closely correlates with the occurrence of MS.

关 键 词：Epstein-Barr virus fluorescent antibody technique herpes virus 4 multiple sclerosis 

分 类 号：R73[医药卫生—肿瘤]