软枣猕猴桃快速繁殖体系生根培养的研究  

Study on Rooting Culture of Actinidia arguta Rapid Propagation System

作　　者：朱君丽 朱磊南 靳松 张萍 李育川 ZHU Junli;ZHU Leinan;JIN Song;ZHANG Ping;LI Yuchuan(College of Agronomy and Life Sciences,Kunming College,Kunming 650000,China;Yunnan Gengxue Agricultural Technology Company,Shangri-La 674400,China)

机构地区：[1]昆明学院农学与生命科学学院,昆明650000 [2]云南耕雪农业科技有限公司,云南香格里拉674400

出　　处：《湖北民族大学学报（自然科学版）》2021年第3期252-255,251,共5页Journal of Hubei Minzu University:Natural Science Edition

基　　金：云南省教育厅科学研究基金项目(2021Y724);昆明学院云南省高校特色生物资源开发与利用重点实验室开放基金项目;云南省优势特色重点学科(生态学)建设项目.

摘　　要：由于软枣猕猴桃在组织培养中不易生根,为提高生根率与成活率,缩短生根时间,提高经济效益,特进行其生根的研究.将1/2 MS、White培养基与不同浓度的NAA、IBA分别组合进行生根培养,根据生根率、最早生根时间、单株平均生根数、平均根长、平均株高以及苗整体生长情况得到最佳基础培养基与激素组合后,再优化活性炭与有机物的添加量.结果表明:活性炭与有机添加物对生根影响较微,且最佳生根培养基配方为1/2 MS+3%蔗糖+0.62%琼脂+0.3 mg/L NAA,pH5.8;在25±2℃、湿度50%~60%、光强2000~3000 lx及12 h/d的条件下,单株平均生根数22.6、根长0.78 cm、株高4.24 cm,植株生长健壮且根系长势优,生根数量多.采用本实验所得的生根培养基配方可用于实际生产中,提高生产效率.Since Actinidia arguta is not easy to take root in tissue culture,we conduct a research on its rooting in order to improve the rooting rate and survival rate,shorten the rooting time,and improve the economic benefits,1/2 MS and White medium are combined with different concentrations of NAA and IBA for rooting culture.The best basic culture can be obtained according to rooting rate,earliest rooting time,average number of roots per plant,average root length,average plant height and overall growth of seedlings.After the base and hormone are combined,the addition amount of activated carbon and organic matter is optimized.The results show that activated carbon and organic additives have little effect on rooting,and the best rooting medium formula is 1/2 MS+3% sucrose+0.62% agar+0.3 mg/L NAA,pH5.8;at 25±2℃,Under the conditions of humidity 50%~60%,light intensity 2000~3000 lx and 12 h/d,the average number of roots per plant is 22.6,the root length is 0.78 cm,and the plant height is 4.24 cm,and the plant grows vigorously and the number of roots grows well.The rooting medium formula obtained in this experiment can be used in actual production to improve production efficiency.

关 键 词：软枣猕猴桃 生根培养 生长素 活性炭 有机添加物 

分 类 号：S318[农业科学—作物栽培与耕作技术]