银杏黄酮降低口腔溃疡复发率的相关免疫学分析  

Immunological study of ginkgo flavone on reducing recurrence rate of oral ulcer

作　　者：张轶涵 张浩然 李婉亭 马文博 ZHANG Yihan;ZHANG Haoran;LI Wanting;MA Wenbo(Qiqihar Medical College,Qiqihar,Heilongjiang 161099,China)

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161099

出　　处：《中国医学工程》2021年第9期14-17,共4页China Medical Engineering

摘　　要：目的分析银杏叶提取物-银杏黄酮对于复发性阿弗他溃疡(RAU)大鼠外周T淋巴细胞亚群和白细胞介素-2(IL-2)的检测结果,探讨应用银杏黄酮对于降低RAU复发率的重要性。方法将50只SD大鼠适应性喂养1周后按随机数字表法分为空白组、模型组、阳性对照左旋咪唑组、低剂量银杏黄酮组[5 mg/(2 mL·d)]、高剂量银杏黄酮组[20mg/(2mL·d)],每组10只。使用生理盐水对正常组与模型组灌胃,使用常规药物左旋咪唑对阳性对照左旋咪唑组灌胃,使用高低剂量银杏黄酮对银杏黄酮组灌胃。观察经过不同药剂灌胃治疗后的大鼠口腔溃疡愈合情况(包括溃疡面面积等指标),然后分别对大鼠T淋巴细胞亚群CD3^(+)、CD4^(+)、CD8^(+)T淋巴细胞水平进行检测并记录。结果各组大鼠IL-2水平与溃疡愈合天数比较,差异有统计学意义(P<0.05)。各组大鼠外周血T淋巴细胞亚群水平比较,差异有统计学意义(P<0.05)。结论银杏黄酮可通过提升大鼠外周血T淋巴细胞水平和IL-2水平治疗RAU,进而可以降低RAU的复发率。【Objective】To analyze the results of detection of peripheral T lymphocyte subsets and IL-2 in rats with recurrent aphthous ulcer by ginkgo biloba extract,and to explore the importance of ginkgo flavone in reducing the recurrence rate of recurrent aphthous ulcer.【Methods】A total of 50 Sprague Dawley rats(180~220 g)were used in this experiment.Forty rats were selected from them to make oral ulcer models.According to the random number table method,the rats were divided into model group,control group,low-dose ginkgo flavone group and high-dose ginkgo flavone group.The normal group and model group were intragastrically administered with normal saline,the control group was given levamisole,and the ginkgo flavone group was given high and low doses of ginkgo flavone.The healing of oral ulcer in rats after intragastric administration of different drugs was observed,including ulcer surface area and other indicators.Then the levels of T lymphocyte subsets(CD 3^(+),CD 4^(+),CD 8^(+)T lymphocytes)were detected and recorded.【Results】The IL-2 levels and the days of ulcer healing of rats in each group were compared,and the difference was statistically significant(P<0.05).There was a statistically significant difference in the levels of T lymphocyte subsets in the peripheral blood of rats in each group(P<0.05).【Conclusion】Ginkgo flavone can treat recurrent aphthous ulcer(RAU)by increasing the level of T lymphocyte and IL-2 in peripheral blood of rats,and then reduce the recurrence rate of RAU.

关 键 词：复发性口腔溃疡 银杏黄酮 淋巴细胞亚群 治疗结果 

分 类 号：R780.1[医药卫生—口腔医学]