微毫秒脉冲电场致细胞DNA转染的仿真研究  

Simulation of DNA Transfection in Cells Underμs/ms Pulsed Electric Field

作　　者：彭豪 张琳 刘欣[1] 郭飞[1] Peng Hao;Zhang Lin;Liu Xin;Guo Fei(Chongqing Key Laboratory of Complex Systems and Bionic Control,Chongqing University of Posts and Telecommunications,Chongqing,400065,China)

机构地区：[1]重庆邮电大学复杂系统与仿生控制重庆市重点实验室,重庆400065

出　　处：《电工技术学报》2021年第18期3829-3840,共12页Transactions of China Electrotechnical Society

基　　金：国家自然科学基金(51507024);重庆自然科学基金(cstc2020jcyj-msxmX0393)资助项目。

摘　　要：为研究微毫秒脉冲电场对细胞摄取DNA的影响规律,基于孔密度、孔径及DNA迁移方程,建立脉冲电场作用下球形细胞DNA转染的数学模型,仿真计算典型脉冲参数作用下胞内DNA浓度的变化特性。仿真结果表明:在单个脉宽100μs、电场强度1kV/cm的脉冲作用下,胞内DNA浓度由0增至7.8×10^(−8)mol/m^(3);而在单个脉宽1ms、电场强度0.5kV/cm的脉冲作用下,胞内DNA浓度增至1.1×10^(−8)mol/m^(3)。同时研究上述两种脉冲组成的双脉冲作用下,其时间间隔(0~1s)以及施加顺序对细胞摄取DNA的影响,结果显示,先施加100μs后再施加1ms脉冲时,不同时间间隔下胞内DNA浓度均为3.45×10^(−7)mol/m^(3);而当改变脉冲施加顺序且脉冲时间间隔小于10ms时DNA浓度为8.9×10^(−8)mol/m^(3),当间隔增至1s时,DNA浓度增至1.68×10^(−7)mol/m^(3)。此外,仿真结果显示,特定条件下DNA摄取浓度与脉冲幅值正相关。仿真结果为脉冲致细胞DNA转染的应用提供了脉冲参数优选指导。In order to study the effect ofμs/ms pulsed electric field on the uptake of DNA by cells,the mathematical model of DNA transfection of spherical cells under the action of pulsed electric field was established based on the equation of pore density,pore radius and DNA migration,and the characteristics of intracellular DNA concentration([DNA]i)with typical pulse parameters were calculated.The simulation results showed that[DNA]i increased from 0 to 7.8×10^(−8)mol/m^(3) with a single pulse of 100μs and a field strength of 1kV/cm,and[DNA]i increased to 1.1×10^(−8)mol/m^(3) with a single pulse of 1ms and a field strength of 0.5kV/cm.Then,the effects of time interval(0~1s)and the order of application on the uptake of DNA under the action of the double pulse composed of the above two pulses were studied.The results showed that[DNA]i was 3.45×10^(−7)mol/m^(3) at different time intervals when 100μs pulse was applied first and then 1ms pulse was applied,[DNA]i was 8.9×10^(−8)mol/m^(3) when the application sequence with the time interval<10ms,and[DNA]i increased to 1.68×10^(−7)mol/m^(3) when the time interval was 1s.In addition,[DNA]i was positively correlated with the pulse amplitude under specific conditions.The simulation results provide parameter optimization guidance for the application of pulse-induce DNA transfection.

关 键 词：脉冲电场 电穿孔 孔径变化 DNA 迁移 双脉冲 

分 类 号：TM836[电气工程—高电压与绝缘技术] Q64[生物学—生物物理学]