LncRNA SNHG17通过诱导上皮间质转化促进结直肠癌转移  

LncRNA SNHG17 promotes the metastasis of colorectal cancer by inducing epithelial-mesenchymal transition

作　　者：耿长辉[1] 张春玲[2] 高萍[3] 何苗[4] 李慧峰[4] 姚旖旎 刘颖新[1] Geng Changhui;Zhang Chunling;Gao Ping;He Miao;Li Huifeng;Yao Yini;Liu Yingxin(General Surgery,the Fifth Affiliated Hospital of Harbin Medical University,Daqing 163316,China;Department of Ultrasound Medicine,Daqing Oilfield General Hospital,Daqing 163001,China;Department of Gastroenterology,Daqing Oilfield General Hospital,Daqing 163001,China;Department of Pathology,Daqing Oilfield General Hospital,Daqing 163001,China;Department of Gastroenterology,the Fifth Affiliated Hospital of Harbin Medical University,Daqing 163316,China)

机构地区：[1]哈尔滨医科大学附属第五医院普外科,大庆163001 [2]大庆油田总医院超声医学科,163000 [3]大庆油田总医院消化内科,163000 [4]大庆油田总医院病理科,163000 [5]哈尔滨医科大学附属第五医院消化内科,大庆163001

出　　处：《中华结直肠疾病电子杂志》2021年第4期367-370,共4页Chinese Journal of Colorectal Diseases(Electronic Edition)

基　　金：黑龙江省科学基金项目(No.H2018017)。

摘　　要：目的探讨长链非编码RNA小核仁RNA宿主基因17(SNHG17)在结直肠癌中的作用及其分子机制。方法采用实时荧光定量PCR(qRT-PCR)技术检测SNHG17在结肠癌细胞系和正常结肠上皮细胞中的表达;通过转染SNHG17质粒或SNHG17-shRNA,过表达或沉默SNHG17,检测其对结直肠癌细胞株迁移及侵袭能力的影响。结果SNHG17在结直肠癌组织和细胞系中表达增加,SNHG17的沉默能抑制结直肠癌细胞迁移和侵袭;SNHG17的上调促进结直肠癌细胞迁移和侵袭。通过检测蛋白水平发现SNHG17促进了结直肠癌细胞的上皮间质转化。结论LncRNA SNHG17在结直肠癌中表达较正常结肠组织增加,通过诱导上皮间质转化促进结直肠癌细胞的迁移和侵袭。Objective To investigate the role of long non-coding RNA small nucleolar RNA host gene 17(SNHG17)and its molecular mechanism in colorectal cancer.Methods Quantitative Realtime PCR(qRT-PCR)was used to detect the expression of SNHG17 in colon cancer cell lines and normal colon epithelial cells;SNHG17 was overexpressed or silenced by transfection with SNHG17 plasmid or SNHG17-shRNA,to detect its effect on the migration and invasion of colorectal cancer cell lines.Results SNHG17 was up-regulated in colorectal cancer tissues and cell lines.The silencing of SNHG17 could inhibit colorectal cancer cell migration and invasion while the up-regulation of SNHG17 promoted colorectal cancer cell migration and invasion.Conclusion LncRNA SNHG17 is highly expressed in colorectal cancer and promotes the migration and invasion of colorectal cancer cells by inducing epithelial-mesenchymal transition.

关 键 词：结直肠肿瘤 长链非编码RNA 小核仁RNA宿主基因17 上皮间质转化 

分 类 号：R735.34[医药卫生—肿瘤]