鸭胚源抗氧化肽TD12对HepG2细胞氧化应激损伤的保护作用  

Protective effect of duck embryo-derived antioxidant peptide TD12 on oxidative stress damage in HepG2 cells

作　　者：吉正梅 张晓春[2] 彭钰迪 王树林[1] 布丽君[2,4] 解华东 JI Zhengmei;ZHANG Xiaochun;PENG Yudi;WANG Shulin;BU Lijun;XIE Huadong(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Chongqing Academy of Animal Sciences,Chongqing 402460,China;College of Biological Engineering,Chongqing University,Chongqing 400044,China;Chongqing Engineering Research Center of Meat Quality Evaluation and Processing,Chongqing 402460,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]重庆市畜牧科学院,重庆402460 [3]重庆大学生物工程学院,重庆400044 [4]重庆市肉质评价与加工工程技术研究中心,重庆402460

出　　处：《食品与发酵工业》2021年第18期141-148,共8页Food and Fermentation Industries

基　　金：重庆市科研院所绩效激励引导专项项目(19539);重庆市科研院所绩效激励引导专项项目(20519)。

摘　　要：该试验旨在研究鸭胚源新型抗氧化肽TD12对HepG2细胞氧化应激损伤的保护作用。采用化学测定法评价TD12体外抗氧化活性;利用H_(2)O_(2)建立HepG2细胞氧化损伤模型,Hoechst 33342染色观察细胞凋亡;CCK-8法检测TD12对细胞的毒性作用及对细胞氧化损伤的保护作用;DCFH-DA荧光探针检测细胞内活性氧含量;试剂盒方法检测细胞抗氧化酶活性;酶联免疫吸附法检测细胞因子含量变化。结果表明,TD12有较强的清除DPPH自由基、ABTS阳离子自由基、羟自由基能力及总抗氧化能力;TD12对HepG2细胞无毒性作用,并且随着TD12浓度的增加能有效降低胞内活性氧积累,对H_(2)O_(2)诱导细胞氧化应激损伤有抑制作用;H_(2)O_(2)诱导损伤细胞中的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性呈浓度依赖性增加;TD12对H_(2)O_(2)诱导氧化损伤的HepG2细胞中乳酸脱氢酶、丙二醛和细胞因子人肿瘤坏死因子-ɑ(TNF-ɑ)、人白细胞介素-8(IL-8)释放有一定抑制作用,同时有效维持人白细胞介素-4(IL-4)与人白细胞介素-10(IL-10)含量的稳定。综上,TD12预处理对HepG2细胞氧化应激损伤有一定的保护作用。This experiment aims to study the protective effect of duck embryo-derived anti-oxidant peptide TD12 on HepG2 cells from oxidative stress damage.The anti-oxidation activity of TD12 in vitro was evaluated by chemical assay.The cells apoptosis was observed by Hoechst 33342 staining.The cytotoxicity and protection of TD12 against oxidative damage were detected by CCK-8 method.And ROS,antioxidant enzyme activity and cytokine content were detected by DCFH-DA fluorescence probe,kit and ELISA method.The results showed that TD12 had a strong scavenging capacity of DPPH and ABTS hydroxyl radical,and had no toxic effect on HepG2 cells.And the accumulation of ROS in the cells was effectively decreased with the increase of TD12 concentration.The activities of SOD,CAT and GSH-Px were increased in a concentration-dependent manner.The TD12 could inhibit the release of LDH,MDA,TNF-ɑand IL-8 in HepG2 cells injured by H_(2)O_(2),and maintain the stability of IL-4 and IL-10.In conclusion,TD12 has a protective effect on oxidative stress injury in HepG2 cells.

关 键 词：鸭胚源抗氧化肽TD12 氧化应激 保护作用 活性氧 抗氧化酶活性 酶联免疫吸附测定 

分 类 号：TS201.4[轻工技术与工程—食品科学]