Src在舌鳞状细胞癌组织中的表达及对舌鳞状细胞癌细胞增殖、迁移和侵袭的作用  

Expression of Src in tongue squamous cell carcinoma tissue and its effects on proliferation,migration and invasion of the carcinoma

作　　者：李军[1] 陈伟程 徐若竹[1] 汤佳臻 LI Jun;CHEN Weicheng;XU Ruozhu;TANG Jiazhen(Department of Stomatology,Longgang District People′s Hospital,Shenzhen 518100,China)

机构地区：[1]深圳市龙岗区人民医院口腔科,广东深圳518100

出　　处：《实用医学杂志》2021年第18期2349-2354,共6页The Journal of Practical Medicine

摘　　要：目的检测Src信号通路相关蛋白Src在舌鳞状细胞癌(TSCC)组织中的表达水平及其对TSCC细胞增殖、迁移和侵袭的作用研究。方法回顾性收集2012年2月至2019年2月在深圳市龙岗区人民医院手术切除的舌鳞癌及其对应癌旁组织20例,制作石蜡切片,采用免疫组织化学法检测TSCC及其癌旁组织Src的表达情况,并分析Src蛋白的表达与临床病理学特征之间的关系。利用shRNA下调TSCC细胞系SCC127中Src的表达,Western blot和RT-PCR验证其抑制效率;利用CCK8、Transwell侵袭实验及划痕实验检测SRC-shRNA干扰后细胞增殖、侵袭迁移能力的影响。结果Src蛋白在口腔癌组织表达显著高于癌旁组织,差异有统计学意义(P<0.01),在TSCC组织中Src蛋白表达与患者的肿瘤分化程度、T分期、临床分期以及淋巴转移密切相关(P<0.05),而与性别和年龄无关(P>0.05)。shRNA下调SCC127中SRC表达后,可以显著抑制SCC127的增殖和侵袭迁移能力。结论Src蛋白在TSCC中高表达,且与TSCC患者预后不良因素相关,下调Src可以降低TSCC细胞的增殖、侵袭和迁移能力,提示其可能是潜在的TSCC诊治靶点。Objective To detect the expression level of Src protein related to Src signal pathway in tongue squamous cell carcinoma(TSCC)and its role in the proliferation,migration and invasion of TSCC tissues.Methods From February 2012 to February 2019,20 cases of tongue squamous cell carcinoma and their corresponding adjacent tissues diagnosed by surgical resection and pathological examinations at our hospital were retrospectively collected.After paraffin sections were made,mmunohistochemistry was used to examine the Src expressions in the carcinoma and its adjacent tissues.The relationship between Src expression and clinicopathological characteristics was analyzed.shRNA was used to down-regulate the expression of SRC in the carcinoma cell line SCC127.Western Blot and RT-PCR were used to verify its inhibitory efficiency.CCK8 and Transwell invasion and migration experiments were done to detect the effects of Src shRNA interference on cell proliferation,invasion and migration.Results The expression of Src on the oral cancer tissues was significantly higher than on the adjacent tissues(P<0.01)and it was significantly related to the patient′s tumor differentiation,T stage,clinical stage,and lymph metastasis(P<0.05),insignificantly to gender and age(P>0.05).Down-regulated expression of Src in SCC127 by shRNA significantly inhibited the proliferation,invasion and migration of SCC127.Conclusion The protein of Src is highly expressed on TSCC tissues,which affects its poor prognosis in patients with tongue cancer.Down-regulating Src can reduce the proliferation,invasion and migration of TSCC,suggesting that it may be a potential target for diagnosis and treatment of tongue cancer.

关 键 词：Src信号通路 Src蛋白 舌鳞状细胞癌 

分 类 号：R739.86[医药卫生—肿瘤]