典型岩溶湿地植物狭叶香蒲对钙胁迫的光合荧光响应  

Photosynthetic fluorescence response of a typical Karst wetland plant Typha angustifolia L.to calcium stress

作　　者：彭博 徐鸣洲 王妍[1] 王艳霞[1] 刘云根[1,2] PENG Bo;XU Mingzhou;WANG Yan;WANG Yanxia;LIU Yungen(College of Ecology and Environment,Southwest Forestry University,Kunming Yunnan 650224;Key Laboratory of Ecological Environment Evolution and Pollution Control in Mountainous Rural Areas of Yunnan Province,Kunming Yunnan 650224)

机构地区：[1]西南林业大学生态与环境学院,云南昆明650224 [2]云南省山地农村生态环境演变与污染治理重点实验室,云南昆明650224

出　　处：《环境污染与防治》2021年第9期1139-1143,共5页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.31660139、No.31760245)。

摘　　要：以供试植物狭叶香蒲(Typha angustifolia L.)为研究对象,利用人工模拟岩溶湿地的试验方法,以添加Ca(NO_(3))_(2)·4H_(2)O的方式设置不同的钙胁迫梯度,考察不同培养时间下狭叶香蒲叶绿素含量及快速叶绿素荧光诱导动力学(OJIP)曲线变化。结果表明:(1)狭叶香蒲在不同钙胁迫处理下叶绿素含量变化显著,培养30 d的狭叶香蒲叶绿素含量与空白对照组相比下降2%~51%,培养60、90 d的狭叶香蒲叶绿素含量总体呈先增加后减少的趋势;2100 mg/kg钙胁迫处理下叶绿素含量显著下降(p<0.05);(2)随着培养时间的延长,不同浓度钙胁迫下OJIP曲线差异变小,狭叶香蒲可通过自身调节来提高光合和捕获光子能力,缓解叶绿素荧光产额下降,继续维持体内正常化学反应和生理机能;(3)总体看来,900~1200 mg/kg的钙胁迫处理下狭叶香蒲在不同培养时间的荧光参数较为稳定,1800~2100 mg/kg的钙胁迫处理下,狭叶香蒲荧光参数随着培养时间的变化出现波动且不稳定,若钙胁迫超过某个阈值后,则狭叶香蒲生长将被明显抑制。In this paper,the Typha angustifolia L.was selected as test plant to cultivate in artificial Karst wetland.The calcium stress levels of Karst wetland was controlled by adding Ca(NO_(3))_(2)·4H_(2)O.The dynamic variation characteristics of Typha angustifolia L.chlorophyll content in different culture periods and dynamic parameters of rapid chlorophyll fluorescence induction(OJIP)curve were investigated.The results showed that:(1)The chlorophyll content of Typha angustifolia L.changed significantly under different levels of calcium stress.For the group of 30-days incubation,calcium stress result in 2%-51%of chlorophyll content decrease compared with the blank control test.For the 60-days and 90-days incubation groups,the chlorophyll content of Typha angustifolia L.presented an increasing first and then decreasing tendency.The chlorophyll content of Typha angustifolia L.decreased significantly(p<0.05)under 2100 mg/kg of calcium stress.(2)With the extension of cultivation time,the difference of OJIP curves under different levels of calcium stress became smaller,and Typha angustifolia L.could improve its photosynthetic and photon capturing capacity through its own regulation to alleviate the decrease of chlorophyll fluorescence yield,and continue to maintain normal chemical reactions and physiological functions in vivo.(3)Overall,the fluorescence parameters of Typha angustifolia L.were stable at different incubation times under calcium stress of 900-1200 mg/kg,while they were fluctuated and unstable with incubation time under calcium stress of 1800-2100 mg/kg.The plant growth would be significantly inhibited if the calcium stress exceeds a certain threshold.

关 键 词：挺水植物 狭叶香蒲 钙胁迫 叶绿素荧光特性 

分 类 号：X173[环境科学与工程—环境科学]