RNF38在非小细胞肺癌组织的表达及其对肺癌细胞增殖、周期和侵袭能力的影响  

Expression of ring finger protein 38 in non-small cell lung cancer and its effect on proliferation,cell cycle and invasion of lung cancer cells

作　　者：王国磊[1] 王洪涛[1] 丁成智[1] 宋静超[1] 姜功前[1] 吴振江[1] 许广辉[1] Wang Guolei;Wang Hongtao;Ding Chengzhi;Song Jingchao;Jiang Gongqian;Wu Zhenjiang;Xu Guanghui(Sixth Extra-thoracic Ward,Henan Chest Hospital,Zhengzhou 450000,China)

机构地区：[1]河南省胸科医院胸外六病区,郑州450000

出　　处：《中华实验外科杂志》2021年第9期1716-1719,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨RNF38在非小细胞肺癌中表达及其对肺癌细胞增殖、周期和迁移能力的影响。方法选取河南省胸科医院2016年8月到2019年1月收治的149例非小细胞肺癌和对应癌旁组织作为研究对象,采用蛋白质组学和蛋白质印迹法(Western blot)分析肿瘤组织和癌旁组织差异表达的蛋白质;采用慢病毒在非小细胞肺癌细胞系A549构建RNF38敲降和对照细胞系(实验组和对照组),采用细胞计数试剂盒(CCK-8)和克隆形成实验分析两组细胞的增殖能力;采用流式细胞术分析两组细胞周期分布;采用划痕实验和Transwell实验分析两组细胞迁移能力。组间计量数据比较采用t检验。结果蛋白质组学显示,非小细胞肺癌组织和癌旁组织中RNF38表达存在显著差异。癌旁组织RNF38蛋白平均表达水平(1.12±0.11)明显低于对照组(3.38±0.23),差异有统计学意义(t=5.891,P<0.05)。RNF38 KD组细胞吸光度(A)值(1.47±0.13)明显低于对照组(2.11±0.17),差异有统计学意义(t=4.908,P<0.05)。克隆形成实验结果显示,RNF38 KD组细胞克隆形成率[(49.37±5.12)%]明显低于对照组[(79.32±8.10)%],差异有统计学意义(t=6.993,P<0.05)。对照组细胞G0/G1期比例[(33.90±3.51)%]明显低于RNF38 KD组[(49.94±5.82)%],差异有统计学意义(t=5.120,P<0.05)。对照组细胞S期比例[(38.40±4.12)%]明显低于RNF38 KD组[(26.33±4.27)%],差异有统计学意义(t=5.113,P<0.05)。RNF38 KD组细胞划痕愈合率[(58.91±6.21)%]明显低于对照组[(84.21±9.24)%],差异有统计学意义(t=6.839,P<0.05)。对照组细胞RNF38表达水平(1.18±0.15)明显高于RNF38 KD组(1.18±0.15),差异有统计学意义(t=3.149,P<0.05)。对照组细胞Runx1蛋白泛素化水平(2.97±0.14)明显高于RNF38 KD组(1.43±0.11),差异有统计学意义(t=2.891,P<0.05)。对照组细胞周期蛋白D3蛋白表达水平(1.87±0.18)明显高于RNF38 KD组(0.89±0.16),差异有统计学意义(t=3.879,P<0.05)。结论 RNF38在非小细胞肺癌中呈高表达Objective To investigate the expression of ring finger protein 38(RNF38)in non-small cell lung cancer(NSCLC)and its effect on cell proliferation,cell cycle and migration.Methods A total of 149 cases of NSCLC tissues and adjacent tissues from August 2016 to January 2019 were selected as the research objects.Proteomics and Western blotting were used to analyze the differentially expressed proteins in tumor tissues and adjacent tissues.Lentivirus was used to construct RNF38 knockdown and control cell lines(experimental group and control group)in NSCLC cell line A549.Cell cycle distribution was analyzed by flow cytometry.The migration ability of the two groups was analyzed by scratch test.T test was used to compare the measurement data between groups.Results There was a significant difference in the expression of RNF38 between NSCLC tissues and adjacent tissues.The average expression level of RNF38 protein in adjacent tissues(1.12±0.11)was significantly lower than that in the control group(3.38±0.23,t=5.891,P<0.05).The absorbance value in RNF38 KD group(1.47±0.13)was significantly lower than that in control group(2.11±0.17,t=4.908,P<0.05).The clone formation rate in RNF38 KD group[(49.37±5.12)%]was significantly lower than that in the control group[(79.32±8.10)%,t=6.993,P<0.05].The ratio of cells in G0/G1 phase in the control group[(33.90±3.51)%]was significantly lower than that in RNF38 KD group[(49.94±5.82)%,t=5.120,P<0.05].The proportion of S phase cells in the control group[(38.40±4.12)%]was significantly lower than that in RNF38 KD group[(26.33±4.27)%,t=5.113,P<0.05].The wound healing rate in RNF38 KD group[(58.91±6.21)%]was significantly lower than that in the control group[(84.21±9.24)%,t=6.839,P<0.05].The expression level of RNF38 in control group(1.18±0.15)was significantly higher than that in RNF38 KD group(1.18±0.15,t=3.149,P<0.05).The ubiquitination level of Runx1 protein in control group(2.97±0.14)was significantly higher than that in RNF38 KD group(1.43±0.11,t=2.891,P<0.05).The expressio

关 键 词：非小细胞肺癌 增殖 周期 迁移 

分 类 号：R734.2[医药卫生—肿瘤]