卡波西样血管内皮瘤体外血管生成三维模型的建立  

An in vitro three-dimension model for angiogenesis of Kaposiform hemangioendothelioma

作　　者：孙正伟 董长宪[2] Sun Zhengwei;Dong Changxian(Department of Radiology,Guangdong Woman and Children Hospital,Guangzhou 511422,China;Department of Hemangioma,Henan Provincial People’s Hospital,Zhengzhou 450003,China)

机构地区：[1]广东省妇幼保健院放射科,广州511422 [2]河南省人民医院血管瘤外科,郑州450003

出　　处：《中华整形外科杂志》2021年第8期894-898,共5页Chinese Journal of Plastic Surgery

基　　金：河南省医学科技攻关计划项目(SB201901070)。

摘　　要：目的探讨体外培养构筑卡波西样血管内皮瘤(KHE)血管生成三维模型的效果及可行性。方法选择2019年4月在河南省人民医院血管瘤科接受手术治疗的1例4个月龄男性KHE患儿,将术中切除的瘤体组织作为实验样本。将处理后的瘤体新鲜标本种植于双层夹心法构筑的纤维凝胶培养体系中,完成KHE血管生成三维模型的建立。潮湿恒温孵育,隔日换液,每日观察生长情况。待模型生长稳定后,取生长情况较好的模型制成冰冻切片行HE染色及免疫组织化学实验验证。结果构筑的模型孵育2~4 d萌生出新生的血管芽,12~16 d血管生长情况趋于稳定,新生血管交错成网状,可观察到明显的管腔样结构,大部分模型于26~30 d后开始凋亡,个别模型直至孵育第54天仍未凋亡。经形态学观察、HE染色、免疫组织化学实验鉴定,证实新生的血管网由KHE血管内皮细胞组成。结论纤维蛋白凝胶培养体系能够完成KHE三维血管生成模型的培养,具有培养时间短、存活时间长、操作简便、实验成本低、易于干涉的特点。Objective To investigate the effect and feasibility of an in vitro three-dimensional model for angiogenesis of Kaposiform hemangioendothelioma(KHE).Methods In April 2019,a 4-month-old boy with KHE received surgery in the department of hemangioma of Henan Provincial People’s Hospital.The tumor tissue was used for the angiogenesis model.Treated tissue was planted in a fibrin gel culture system constructed by a double-layer sandwich method to establish a three-dimensional angiogenesis model.Optimum humidity and constant temperature was provided for the culture system,and the medium was changed every other day.The growth of model was observed every day.When the model became stable,well-grown models were made into frozen sections for HE and immunohistochemistry staining.Results The model formed newborn vascular buds within 2-4 days and became stable in 12-16 days.The newborn vessels staggered into networks and showed obvious lumen-like structure.Apoptosis started after 26-30 days in most models,while no apoptosis appeared until 54 days in some ones.Morphological observation,HE staining,and immunohistochemistry examination confirmed that KHE endothelia have formed the new vessels.Conclusions The culture system of fibrin gel is applicable for three-dimensional angiogenesis model of Kaposiform hemangioendothelioma.It is characterized with short culture time,long survival time,simple operation,low cost,and easy intervention.

关 键 词：内皮细胞 体外培养技术 卡波西样血管内皮瘤 

分 类 号：R732.2[医药卫生—肿瘤]