槲皮素联合顺铂调控PI3K/Akt信号通路对肺癌A549/DDP细胞增殖与凋亡的影响  

Effects of quercetin combined with cisplatin on PI3K/Akt signaling pathway on proliferation and apoptosis of lung cancer A549/DDP cells

作　　者：赵纯 管养洪 刘艳[3] 张杰[4] ZHAO Chun;GUAN Yanghong;LIU Yan(Department of Nosocomial Infection Management,Zhejiang Quhua Hospital,Quzhou 324004.China.)

机构地区：[1]浙江衢化医院医院感染管理科,浙江衢州324004 [2]衢州市柯城区中医医院药剂科 [3]浙江衢化医院功能科 [4]浙江衢化医院药剂科

出　　处：《全科医学临床与教育》2021年第9期781-784,共4页Clinical Education of General Practice

基　　金：衢州市科技项目(2019098)。

摘　　要：目的研究槲皮素联合顺铂对肺癌A549/DDP细胞增殖与凋亡的影响,并探讨磷脂酰肌醇-3-激酶(PI3K/丝氨酸-苏氨酸蛋白激酶(Akt)信号通路的作用机制。方法将对数生长期人肺腺癌耐顺铂细胞株A549/DDP分为槲皮素+顺铂组(给予160μmol/L浓度的槲皮素和25μmol/L浓度的顺铂)、顺铂组(给予25μmol/L浓度的顺铂)、槲皮素组(给予160μmol/L浓度的槲皮素)和对照组(不经药物干预)。CCK-8法观察细胞增殖抑制率;流式细胞术计算细胞凋亡率;Western blot检测p-PI3K、p-Akt蛋白水平的表达。结果各组A549/DDP细胞增殖抑制率和细胞凋亡率比较,差异均有统计学意义(F分别=12.26、163.10,P均<0.05)。槲皮素组的细胞增殖抑制率和细胞凋亡率高于对照组,差异均有统计学意义(t分别=20.58、8.54,P均<0.05);顺铂组的细胞增殖抑制率和细胞凋亡率高于槲皮素组,差异均有统计学意义(t分别=2.17、6.32,P均<0.05);槲皮素+顺铂组的细胞增殖抑制率和细胞凋亡率高于顺铂组,差异均有统计学意义(t分别=2.54、5.78,P均<0.05)。各组A549/DDP细胞p-PI3K/PI3K、p-Akt/Akt蛋白表达水平比较,差异均有统计学意义(F分别=47.88、41.30,P均<0.05)。槲皮素组的p-PI3K/PI3K、p-Akt/Akt蛋白表达水平较对照组明显降低,差异均有统计学意义(t分别=4.76、4.09,P均<0.05);顺铂组的p-PI3K/PI3K、p-Akt/Akt蛋白表达水平较槲皮素组明显降低,差异均有统计学意义(t分别=2.38、4.34,P均<0.05);槲皮素+顺铂组的p-PI3K/PI3K、p-Akt/Akt蛋白表达水平低于顺铂组,差异均有统计学意义(t分别=7.05、10.28,P均<0.05)。结论槲皮素与顺铂联用能够抑制A549/DDP细胞增殖,诱导A549/DDP细胞凋亡,其机制与PI3K/Akt信号通路有关。Objective To investigate the effects of quercetin combined with cisplatin on the proliferation and apoptosis of lung cancer A549/DDP cells,and to explore the mechanism of PI3K/Akt signaling pathway.Methods The logarithmi-cally growing human lung adenocarcinoma cell line A549/DDP was divided into quercetin combined with cisplatin group(given 160μmol/L quercetin and 25μmol/L cisplatin),cisplatin group(given 25μmol/L cisplatin),quercetin group(given 160μmol/L quercetin)and control group(without drug intervention).The inhibitory rate of cell proliferation was observed by CCK-8 method.The apoptosis rate was calculated by flow cytometry.The protein levels of p-PI3K and p-Akt were detected by Western blot.Results The cell proliferation inhibition rate and apoptosis rate of A549/DDP cells were significantly different among all groups(F=12.26,163.10,P<0.05).The inhibitory rate of cell proliferation and apoptosis in quercetin group were higher than those in control group(t=20.58,8.54,P<0.05).The inhibitory rate of cell prolifer-ation and apoptosis in cisplatin group were higher than those in quercetin group(t=2.17,6.32,P<0.05).The proliferation inhibition rate and apoptosis rate in quercetin com-bined with cisplatin group were higher than those in cisplatin group(t=2.54,5.78,P<0.05).The protein expression levels of p-PI3K/PI3K and p-Akt/Akt in A549/DDP cells were significantly different among all groups(F=47.88,41.30,P<0.05).The expression levels of p-PI3K/PI3K and p-Akt/Akt in quercetin group were sig-nificantly lower than those in control group(t=4.76,4.09,P<0.05).The protein expression levels of p-PI3K/PI3K and p Akt/Akt in cisplatin group were significantly lower than those in quercetin group(t=2.38,4.34,P<0.05).The protein ex-pression levels of p-PI3K/PI3K and p-Akt/Akt in quercetin combined with cisplatin group were significantly lower than those in cisplatin group(t=7.05,10.28,P<0.05). Conclusion Quercetin combined with cisplatin can inhibit the prolif-eration of A549/DDP cells and induce the apoptosis of A549/D

关 键 词：槲皮素 顺铂 PI3K/AKT信号通路 肺癌A549/DDP细胞 增殖 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]