抗虫耐除草剂玉米对大鼠的亚慢性毒性研究  

Sub-chronic toxicity test of genetically modified insect-and herbicide-resistant corn in rats

作　　者：周殿明[1] 张大龙[1] 张静[1] 何宁[1] 高峥 张倩[1] 周晓丽[1] 钱智勇[1] ZHOU Dianming;ZHANG Dalong;ZHANG Jing;HE Ning;GAO Zheng;ZHANG Qian;ZHOU Xiaoli;QIAN Zhiyong(Department of Toxicology,Tianjin Center for Disease Control and Prevention,Tianjin 300011,China)

机构地区：[1]天津市疾病预防控制中心毒理科,天津300011

出　　处：《癌变．畸变．突变》2021年第5期383-387,392,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家“十三五”转基因重大专项(2016ZX08011005)。

摘　　要：目的:研究大鼠在90 d内经口重复喂饲含抗虫基因cry1Ab和cry3Bb、耐除草剂基因cp4epsps、高维生素基因ZmHPT和ZmTMT的玉米引起的毒性效应。方法:140只6周龄的Wistar大鼠,雌雄各半,按照不同饲料随机分为7组:基础饲料对照组,对照玉米低、中、高剂量组,转基因玉米低、中、高剂量组。分别喂饲含相应玉米的饲料90 d,观察体质量、进食量、血液生化指标、脏器系数及病理学检查。结果:各组大鼠试验周期内生长正常,部分转基因玉米组大鼠和对照玉米组大鼠体质量、进食、血液生化指标、脏器系数与基础饲料对照组大鼠比较,差异具有统计学意义(P<0.05),部分转基因玉米组大鼠体质量、进食、血液生化指标、脏器系数与对照玉米组大鼠比较,差异具有统计学意义(P<0.05),但是均无生物学意义。主要脏器组织病理学检查未发现明显异常。结论:在本实验条件下,未发现该转基因玉米对大鼠有亚慢性毒性作用。OBJECTIVE:The study investigated toxic effects of sub-chronic oral exposures for 90 days in rats to genetically modified(GM)corn with insect-resistant gene cry1Ab and cry3Bb,herbicide-resistant gene cp4epsps,high vitamin gene ZmHPT and ZmTMT.METHODS:6-week-old Westar rats,half males and half females,were randomly divided into 7 groups with 20 per group according to weight:basic feed control group,non-GM corn groups(low-,medium-and high-doses),GM corn experimental groups(low-,medium-and high-doses).Rats were fed the different corns for 90 consecutive days.Then,they were observed for changes in body weight,food intake,blood biochemical indicators,organ coefficients and pathological features.RESULTS:Rats in each dose group grew normally during the test cycle.The body weight,food intake,blood biochemical indexes,organ coefficients of some GM corn and non-GM corn groups were significantly different from those in the control animals(P<0.05).In addition,the differences were also significantly different between the GM corn compared with the non-GM corn groups(P<0.05).However,no significant abnormalities were found in the pathology examinations of some main organ tissues.CONCLUSION:The experimental results show that sub-chronic exposure to the genetically modified corns did not lead to obvious pathological changes in rats.

关 键 词：转基因玉米 亚慢性毒性 大鼠 90d喂养试验 

分 类 号：R394.6[医药卫生—医学遗传学]