2型人类表皮生长因子受体基因对结直肠癌自然杀伤细胞92局部浸润的影响及机制  

Effect and mechanism of HER2 gene on local infiltration and Wnt/β-catenin signal of NK cells in colorectal cancer

作　　者：谭定春 秦惠何 TAN Dingchun;QIN Huihe(General Medicine,Shenzhen Longhua District People’s Hospital,Shenzhen,Guangdong 518110,China)

机构地区：[1]深圳市龙华区人民医院,广东深圳518110

出　　处：《安徽医药》2021年第10期2098-2101,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨抑制2型人类表皮生长因子受体(HER2)基因表达对结直肠癌自然杀伤细胞92(NK92)细胞局部浸润的影响及机制。方法本研究起止时间为2018年5—11月。结直肠癌细胞(SW480、SW620和HT29)细胞及人正常肠上皮细胞(NCM460)购自美国模式培养物保藏所。Western blotting检测上述细胞中HER2的蛋白表达。Western blotting及ELISE检测HER2的特异性小干扰RNA(si-HER2)转染HT29细胞效率。Transwell小室检测重组HER2及肿瘤培养上清对NK92细胞迁移指数的影响。Transwell小室检测及Western blotting检测si-HER2对NK92细胞迁移指数及β-catenin表达影响。结果3株结直肠癌细胞HER2表达均明显高于在NCM460细胞表达[(0.104±0.011)、(0.141±0.015)、(0.243±0.018)比(0.018±0.003)]。对照组HER2蛋白及HER2含量均明显高于si-HER2组[(0.467±0.046)比(0.101±0.012),(125.3±10.1)pg/mL比(74.7±4.6)pg/mL]。重组HER2均能抑制NK92的迁移,促进β-catenin蛋白表达,而转染si-HER2可提高NK92迁移,且抑制β-catenin蛋白表达。结论抑制HER2基因表达可增强结直肠癌NK92细胞局部浸润,机制与下调Wnt/β-catenin信号有关。Objective To explore the mechanism of inhibiting the expression of human epidermal growth factor receptor type 2(HER2)gene on the infiltration of Natural killer cell 92(NK92)cells in colorectal cancer.Methods The start and end time of this study was from May 2018 to November 2018.C.olorectal cancer cells(SW480,SW620 and HT29)and human normal intestinal epithelial cells(NCM460)were purchased from American Type Culture Collection.The expression of HER2 protein was detected by Western blotting.Western blotting and ELISA were used to detect the transfection efficiency of HER2-specific small interfering RNA(si-HER2)into HT29 cells.The effect of recombinant HER2 and tumor culture supernatant on NK92 cell migration index was detected by Transwell cell assay.Transwell chamber test and Western blotting test were used to detect the effect of si-HER2 on migration index and beta-catenin expression of NK92 cells.Results The expression of HER2 in 3 colorectal cancer cells was significantly higher than that in NCM460 cells[(0.018±0.003)vs.(0.104±0.011),(0.141±0.015),(0.243±0.018)].The expression of HER2[(0.467±0.046)vs.(0.101±0.012)]and HER2 content[(125;3±10.1)pg/mL vs.(74.7±4.6)pg/mL]in si-HER2 group were significantly lower than those in control group.Recombinant HER2 could inhibit the migration of NK92[(1.00±0.10)vs.(0.63±0.09)],and promoteβ-catenin protein expression.However,transfection of si-HER2 could significantly enhance the migration of NK92 and inhibit the expression ofβ-catenin protein.Conclusion Inhibition of HER2 gene expression can enhance the local invasion of colorectal cancer NK92 cells,and the mechanism is related to down-regulation of Wnt/β-catenin signal.

关 键 词：结直肠肿瘤 基因 ERBB-2 受体 自然杀伤细胞 Wnt信号通路 趋化运动 NK细胞 Β连环素 

分 类 号：R735.34[医药卫生—肿瘤]