Chromatin remodeler INO80 mediates trophectoderm permeability barrier to modulate morula-to-blastocyst transition  

作　　者：Zu-Bing Cao Di Gao Hui-Qun Yin Hui Li Teng-Teng Xu Meng-Ya Zhang Xin Wang Qiu-Chen Liu Ye-Lian Yan Yang-Yang Ma Tong Yu Yun-Sheng Li Yun-Hai Zhang 

机构地区：[1]Anhui Province Key Laboratory of Local Livestock and Poultry,Genetical Resource Conservation and Breeding,College of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui 230036,China [2]Reproductive Medicine Center,901st Hospital,Hefei,Anhui 230031,China

出　　处：《Zoological Research》2021年第5期562-573,共12页动物学研究（英文）

基　　金：supported by the Anhui Provincial Natural Science Foundation(1908085MC97,2008085MC85);National Natural Science Foundation of China(31802059,31902226);Hefei Innovation and Entrepreneurship Support Plan for Returnee Scholar(03082009);Anhui Provincial Innovation and Entrepreneurship Support Plan for Returnee Scholar(2020LCX015)。

摘　　要：Inositol requiring mutant 80(INO80)is a chromatin remodeler that regulates pluripotency maintenance of embryonic stem cells and reprogramming of somatic cells into pluripotent stem cells.However,the roles and mechanisms of INO80 in porcine preimplantation embryonic development remain largely unknown.Here,we show that INO80 modulates trophectoderm epithelium permeability to promote porcine blastocyst development.The INO80 protein is highly expressed in the nuclei during morula-toblastocyst transition.Functional studies revealed that RNA interference(RNAi)-mediated knockdown of INO80 severely blocks blastocyst formation and disrupts lineage allocation between the inner cell mass and trophectoderm.Mechanistically,singleembryo RNA sequencing revealed that INO80 regulates multiple genes,which are important for lineage specification,tight junction assembly,and fluid accumulation.Consistent with the altered expression of key genes required for tight junction assembly,a permeability assay showed that paracellular sealing is defective in the trophectoderm epithelium of INO80 knockdown blastocysts.Importantly,aggregation of 8-cell embryos from the control and INO80 knockdown groups restores blastocyst development and lineage allocation via direct complementation of the defective trophectoderm epithelium.Taken together,these results demonstrate that INO80 promotes blastocyst development by regulating the expression of key genes required for lineage specification,tight junction assembly,and fluid accumulation.

关 键 词：INO80 BLASTOCYST TROPHECTODERM Tight junction Permeability 

分 类 号：Q132[生物学—普通生物学]