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作 者:魏玲玲 王玉林[1,3] 原红军[1,3] 旺姆[2] 曾兴权[1,3] Wei Lingling;Wang Yulin;Yuan Hongjun;Wang Mu;Zeng Xingquan(State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa,850002;Tibet Agriculture and Animal Husbandry University,Linzhi,86000;Research Institute of Agriculture,Tibet Academy of Agriculture and Animal Husbandry Sciences,Lhasa,850002)
机构地区:[1]西藏自治区农牧科学院,省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,拉萨850002 [2]西藏大学农牧学院,林芝860000 [3]西藏自治区农牧科学院农业研究所,拉萨850002
出 处:《分子植物育种》2021年第17期5581-5590,共10页Molecular Plant Breeding
基 金:国家重点研发项目(2018YFD1000703);西藏财政专项基金(XZNKY-2019-C-051)共同资助
摘 要:白粉病是麦类作物中最具破坏性的病害之一,对谷物产量和品质造成严重损失。为明确西藏青稞对白粉病的抗病分子机制,本研究对两个青稞品种‘甘农大7号’(G7:高抗)和‘藏青13’(Z13:高感)进行白粉菌(Blumeria graminis f.sp.hordei,Bgh)接种,研究其基因的差异表达模式。对G7和Z13白粉菌接种前后的差异基因比较发现,钙依赖蛋白激酶、谷胱甘肽S-转移酶、纤溶酶原激活物抑制剂1、丝氨酸型内肽酶和MADS-box转录因子的编码基因在G7中表达水平较高,表明它们可能在抗Bgh中发挥作用。G7高表达的基因主要参与了离子通道、钙依赖蛋白激酶、植物激素途径等生物过程。另外,16个lncRNA及其21个候选靶基因表现出协同表达模式,这些表达模式丰富了植物激素信号转导和离子结合的过程。本研究结果对后续青稞及其近缘物种抗白粉病的分子机制研究具有一定的参考价值。Powdery mildew,known as one of the most devastating diseases in cereal crops,causes serious losses in grain yield and quality.In order to clarify the pathogenic molecular mechanism of qingke barley to powdery mildew.In the present study,the DEGs expression patterns of Tibetan barley(also called qingke)cultivars G7(showed complete penetration resistance to powdery mildew)and Z13(showed complete penetration sensitivity to powdery mildew)were compared after inoculation with Blumeria graminis f.sp.hordei(Bgh).Comparing the differential genes of G7 and Z13 before and after inoculation,it was found that the expression levels of calcium-dependent protein kinase,glutathione S-transferase,plasminogen activator inhibitor 1,serine-type endopeptidase,and MADS-box transcription factor were expressed at higher levels in the G7,indicating their possible roles in the resistance to Bgh.The highly expressed genes in G7 take a part in the biological processes including ion channels,calcium-dependent protein kinase,and plant hormone pathway.Sixteen lncRNAs and their 21 candidate target genes were identified to exhibit coordinated expression patterns,which were enriched in plant hormone signal transduction and ion binding processes.This study casts an insight into the molecular mechanisms to control powdery mildew in barley and relative species.
分 类 号:S435.121.46[农业科学—农业昆虫与害虫防治]
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