马铃薯野生种冷驯化能力的蛋白组学分析  

Proteomic Analysis of the Cold Acclimation Ability of Wild Potato Species

作　　者：何天久 李飞[1] 吴巧玉 He Tianjiu;Li Fei;Wu Qiaoyu(Institute of Biotechology,Guizhou Academy of Agricultural Sciences,Guiyang,550006)

机构地区：[1]贵州省农业科学院生物技术研究所,贵阳550006

出　　处：《分子植物育种》2021年第17期5591-5598,共8页Molecular Plant Breeding

基　　金：贵州省科学技术基金项目(黔科合基础〔2018〕1152);贵州省创新人才团队建设项目(黔科合人才团队〔2020〕5002);贵州省第五批创新人才基地建设项目(黔人领发〔2016〕22)共同资助。

摘　　要：为研究马铃薯冷驯化机理,通过同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantification,iTRAQ)分析了冷驯化过程中(0,4和12 d)马铃薯野生种Solaum acaule(W3,具备冷驯化能力)和S.cardiophyllum(Cph12,无冷驯化能力)的蛋白质组变化。结果表明,在W3和Cph12中筛选的可信蛋白质数量分别为2126和1044;在冷驯化早期分别筛选到103与56个差异表达蛋白,后期分别筛选到330和155个;富集到与耐寒性或冷驯化直接相关的途径27条,例如膜脂质成分的调节,渗透保护剂的合成,抗氧化代谢,能量代谢和光合作用等。本研究结果为深入研究马铃薯冷驯化的分子机制提供理论指导,也可为马铃薯抗寒基因挖掘及分子育种提供参考。At the aim of revealing the mechanism of potato cold domestication,the proteome changes of wild potato species Solanum acaule(W3,with cold acclimation ability)and S.cardiophyllum(Cph12,non-cold acclimation ability)after cold-acclimation treatment for 0,4 and 12 d were analyzed through isobaric tags for relative and absolute quantification.The results showed that the number of trusted proteins filtered from W3 and Cph12 were 2126 and 1044 respectively;The number of differentially expressed proteins screened in W3 and Cph12 in the early stage of cold acclimation were 103 and 56,and that in the late stage were 330 and 155.There were 27 pathways directly related to cold tolerance or cold acclimation enriched in W3 and Cph12,such as regulation of Membrane Lipids,synthesis of Osmotic protectants,antioxidant metabolism,energy metabolism and photosynthesis.The results of this study provide theoretical guidance for further study on the molecular mechanism of cold acclimation of potato,and also provide reference for searching cold resistance genes and potato molecular breeding.

关 键 词：马铃薯 冷驯化 蛋白组学 差异表达蛋白 

分 类 号：S532[农业科学—作物学]