HPV E6/E7 mRNA检测联合TCT检查在宫颈病变早期筛查中的应用价值  

Application value of HPV E6/E7 mRNA combined with TCT in early screening of cervical lesions

作　　者：陈雪峰 王芝英 邓达 顾卉晴 韩琼波 林剑 CHEN Xue-feng;WANG Zhi-ying;DENG Da(Department of Gynecology,Medical Health Group of First People’s Hospital of Xiangshan County,Ningbo 315700,China)

机构地区：[1]浙江省象山县第一人民医院医疗健康集团妇科,315700

出　　处：《中国实用医药》2021年第27期56-58,共3页China Practical Medicine

摘　　要：目的探究人乳头瘤病毒(HPV)E6/E7信使核糖核酸(mRNA)检测联合液基薄层细胞学检测(TCT)在宫颈病变早期筛查中的应用价值。方法130例宫颈病变患者为研究对象,均进行HPV E6/E7 mRNA检测与TCT检查,以阴道镜下活检结果为金标准,观察并记录所有患者分别使用HPV E6/E7 mRNA检测与TCT检查的宫颈病变情况。对比HPV E6/E7 mRNA检测、TCT检查、HPV E6/E7 mRNA检测与TCT检查联合检测三种检测方式的灵敏度与特异度。结果HPV E6/E7 mRNA检测与TCT检查宫颈病变阳性检出率随病程增加而增加。HPV E6/E7 mRNA检测与TCT检查的宫颈癌阳性检出率均高于宫颈炎、宫颈上皮内瘤变轻度不典型增生(CINⅠ级)、宫颈上皮内瘤变中度不典型增生(CINⅡ级)、宫颈上皮内瘤变重度不典型增生(CINⅢ级)的阳性检出率,差异均具有统计学意义(P<0.05)。HPV E6/E7 mRNA检测与TCT检查联合检测的灵敏度为97.89%、特异度为94.29%,均高于HPV E6/E7 mRNA单独检测的78.95%、77.14%和TCT单独检测的86.32%、71.43%,差异具有统计学意义(P<0.05)。结论随着宫颈病程的不断增加,HPV E6/E7 mRNA检测、TCT检查的阳性检出率也持续增加,而且HPV E6/E7 mRNA检测与TCT检查的联合检测在宫颈病变的早期筛查中具有更高的灵敏度及特异度,能够为临床治疗提供更为全面、科学的检测依据,应在妇科检查中进行应用与推广。Objective To investigate the application value of human papillomavirus(HPV)E6/E7 messenger ribonucleic acid(mRNA)combined with thinprep cytologic test(TCT)in early screening of cervical lesions.Methods There were 130 patients with cervical lesions as the subjects of the study,and all were tested for HPV E6/E7 mRNA and TCT.Taking the biopsy results under colposcopy as the gold standard,the cervical lesions of all patients were observed and recorded by HPV E6/E7 mRNA and TCT.The sensitivity and specificity of HPV E6/E7 mRNA,TCT alone,and combined detection of HPV E6/E7 mRNA and TCT were compared.Results The positive detection rate of HPV E6/E7 mRNA and TCT for cervical lesions increased with the course of the disease.The positive detection rates of HPV E6/E7 mRNA and TCT for cervical cancer were higher than those for cervicitis,cervical intraepithelial neoplasia gradeⅠ(CINⅠ),cervical intraepithelial neoplasia gradeⅡ(CINⅡ),and cervical intraepithelial neoplasia gradeⅢ(CINⅢ),and the differences were statistically significant(P<0.05).The sensitivity and specificity of the combined detection of HPV E6/E7 mRNA and TCT were 97.89%and 94.29%,respectively,which were higher than 78.95%and 77.14%of HPV E6/E7 mRNA alone,and 86.32%and 71.43%of TCT alone.All the differences were statistically significant(P<0.05).Combination With the increasing of cervical disease course,the positive detection rate of HPV E6/E7 mRNA and TCT will continue to increase,and the combined detection of HPV E6/E7 mRNA and TCT has higher sensitivity and specificity in the early screening of cervical lesions,which can provide more comprehensive and scientific detection basis for clinical treatment,and should be applied and promoted in gynecological examination.

关 键 词：人乳头瘤病毒E6/E7 液基薄层细胞学检测 宫颈病变 早期筛查 应用价值 

分 类 号：R737.33[医药卫生—肿瘤]