c-Myc在人胃癌组织中的表达及其对胃癌细胞增殖、迁移和侵袭的影响  

Expression of c-Myc in human gastric cancer tissues and its effect on proliferation,migration and invasion of gastric cancer cells

作　　者：刘德仁 丁闯 侍孝红 刘加宁 乔彤 LIU Deren;DING Chuang;SHI Xiaohong;LIU Jianing;QIAO Tong(Department of Gastroenterology,Suqian People's Hospital of Nanjing Drum Tower Hospital Group(Suqian Hospital Affiliated to Xuzhou Medical University),Jiangsu Suqian 223800,China;General Surgery,Suqian People's Hospital of Nanjing Drum Tower Hospital Group(Suqian Hospital Affiliated to Xuzhou Medical University),Jiangsu Suqian 223800,China;Department of Pathology,Suqian People's Hospital of Nanjing Drum Tower Hospital Group(Suqian Hospital Affiliated to Xuzhou Medical University),Jiangsu Suqian 223800,China;Department of Vascular Surgery,Nanjing Drum Tower Hospital(Drum Tower Hospital Affiliated to Nanjing University School of Medicine),Jiangsu Nanjing 210008,China)

机构地区：[1]南京鼓楼医院集团宿迁市人民医院(徐州医科大学附属宿迁医院)消化科,江苏宿迁223800 [2]南京鼓楼医院集团宿迁市人民医院(徐州医科大学附属宿迁医院)普通外科,江苏宿迁223800 [3]南京鼓楼医院集团宿迁市人民医院(徐州医科大学附属宿迁医院)病理科,江苏宿迁223800 [4]南京鼓楼医院(南京大学医学院附属鼓楼医院)血管外科,江苏南京210008

出　　处：《现代肿瘤医学》2021年第20期3526-3531,共6页Journal of Modern Oncology

基　　金：江苏省第十四批“六大人才高峰”高层次人才选拔培养资助计划(编号:WSN-134)。

摘　　要：目的:探讨c-Myc在人胃癌组织中的表达及其对胃癌细胞增殖、迁移和侵袭的影响。方法:免疫组化和qRT-PCR检测胃癌组织及其癌旁组织中c-Myc的表达;qRT-PCR和Western blot检测人胃黏膜细胞GES-1和人胃癌细胞HGC-27、AGS、SGC-7901中c-Myc的表达;HGC-27细胞转染siRNA-NC和siRNA-c-Myc。48 h后,CCK-8检测细胞增殖能力;流式细胞术检测细胞周期;Transwell实验检测细胞迁移和侵袭;Western blot检测细胞低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、CXC亚家族受体4(cysteine X cysteine receptor 4,CXCR4)和基质细胞衍生因子(stromal cell derived factor-1,SDF-1)蛋白表达。结果:与癌旁组织相比,胃癌组织中c-Myc mRNA表达和免疫组化评分升高(P<0.05)。与GES-1组相比,HGC-27组、SGC-7901组和AGS组中c-Myc mRNA和蛋白表达均升高(P<0.05)。与siRNA-NC组相比,siRNA-c-Myc组细胞在24 h、48 h和72 h增殖能力减弱(P<0.05),G 0/G 1期细胞比例升高(P<0.05),G 2/M和S期细胞比例降低(P<0.05),迁移细胞数和侵袭细胞数减少(P<0.05),HIF-1α、CXCR4和SDF-1蛋白表达降低(P<0.05)。结论:敲低c-Myc可抑制胃癌细胞增殖、迁移和侵袭,诱导细胞周期阻滞,该作用可能是通过抑制HIF-1α/CXCR4通路实现的。Objective:To investigate the expression of c-Myc in human gastric cancer tissues and its effect on proliferation,migration and invasion of gastric cancer cells.Methods:Immunohistochemistry and qRT-PCR were used to detect the expression of c-Myc in gastric cancer tissues and adjacent tissues.qRT-PCR and Western blot were used to detect the expression of c-Myc in human gastric mucosal cells GES-1,human gastric cancer cells HGC-27,AGS and SGC-7901.HGC-27 cells were transfected with siRNA-NC and siRNA-c-Myc.After 48 hours,CCK-8 was used to detect cell proliferation.Flow cytometry was used to detect cell cycle.Transwell experiment was used to detect cell migration and invasion.Western blot was used to detect the protein expression of hypoxia inducible factor-1α(HIF-1α),cysteine X cysteine receptor 4(CXCR4)and stromal cell derived factor-1(SDF-1).Results:Compared with adjacent tissues,c-Myc mRNA expression and immunohistochemical score in gastric cancer tissues were increased(P<0.05).Compared with the GES-1 group,the expressions of c-Myc mRNA and protein in the HGC-27 group,SGC-7901 group and AGS group were increased(P<0.05).Compared with the siRNA-NC group,the proliferation ability of cells in the siRNA-c-Myc group decreased at 24 h,48 h and 72 h(P<0.05),the ratio of cells in G 0/G 1 phase increased(P<0.05),and the proportion of cells in G 2/M and S phases decreased(P<0.05),the number of migrating cells and the number of invasive cells decreased(P<0.05),and the protein expression of HIF-1α,CXCR4 and SDF-1 decreased(P<0.05).Conclusion:Knockdown of c-Myc can inhibit the proliferation,migration and invasion of gastric cancer cells,and induce cell cycle arrest.This effect may be achieved by inhibiting the HIF-1α/CXCR4 pathway.

关 键 词：C-MYC 胃癌 HIF-1Α CXCR4 

分 类 号：R735.2[医药卫生—肿瘤]