高灵敏度hMAM-QDs脂质体结合CK19双标记法检测乳腺癌循环肿瘤细胞的临床研究  

High-sensitivity hMAM-QDs liposomes combined with CK19 double-labeled method detection for circulating tumor cells in breast cancer and early clinical verification

作　　者：乔晶晶 尤伟名 张静[2] 周昊[2] 郑田玉 张吉瑞 靳小倩 杨光[2] QIAO Jingjing;YOU Weiming;ZHANG Jing;ZHOU Hao;ZHENG Tianyu;ZHANG Jirui;JIN Xiaoqian;YANG Guang(Department of Oncology,BenQ Medical Center Affiliated to Nanjing Medical University,Jiangsu Nanjing 210005,China;Department of Oncology,Suzhou BenQ Medical Center Affiliated to Nanjing Medical University,Jiangsu Suzhou 215010,China)

机构地区：[1]南京医科大学附属明基医院肿瘤科,江苏南京210005 [2]南京医科大学附属明基医院苏州院区肿瘤科,江苏苏州215010

出　　处：《现代肿瘤医学》2021年第20期3532-3537,共6页Journal of Modern Oncology

基　　金：江苏省苏州市科技局资助项目(编号:SS201762);南京医科大学科技发展基金项目(编号:NMUB2018188)。

摘　　要：目的:评估细胞角蛋白19(CK19)单标记法和CK19结合人乳球蛋白-量子点(hMAM-QDs)双标记法检测乳腺癌循环肿瘤细胞(circulating tumor cells,CTCs)的临床价值。方法:纳米脂质体(PL)技术用于构建hMAM-PL(QDs);借助免疫磁珠分离技术,使用上皮细胞黏附分子(EpCAM)抗体分离乳腺癌CTCs。结果:hMAM-PL(QDs)[平均粒径(102.6±1.1)nm,多分散指数0.047]可以显著提高实验中的检测灵敏度。此外,CK19-FITC与hMAM-PL(QDs)双标记方法相结合可有效区分分离的CTCs,提高准确性,并减少乳腺癌检测中的假阳性。CTCs编号、图像与临床特征以及病理状况高度一致。结论:CK19结合hMAM-PL(QDs)双标记方法检测比CK19单标记方法更准确。双标记方法可以显著减少假阳性结果,对于早期乳腺癌的诊断、治疗和预后具有重要意义。Objective:To evaluate the clinical value of detecting circulating tumor cells(CTCs)in breast cancer by cytokeratin 19(CK19)single-labeled method and CK19 combined with human mammaglobin-quantum dots(hMAM-QDs)double-labeled method.Methods:Nanopolymeric liposome(PL)technology was used to build hMAM-PL(QDs),and epithelial cell adhesion molecule(EpCAM)antibody immune magnetic ball separation technology was applied to separate breast CTCs.Results:hMAM-PL(QDs)[average particle size(102.6±1.1)nm with polydispersity index 0.047]can obviously improve detection sensitivity in cell experiments.Furthermore,CK19-FITC combined with hMAM-PL(QDs)double-labeled method can efficiently distinguish separated CTCs,increase accuracy,and decrease false positive in breast cancer detection.The CTCs numbers and images were highly consistent with clinical characteristics and pathology conditions.Conclusion:The CK19 combined with hMAM-PL(QDs)double-labeled method detection is more accurate than the CK19 single-labeled method.The double-labeled method can significantly decrease false positive results,and is useful for early breast cancer diagnosis,treatment and prognosis.

关 键 词：循环肿瘤细胞 乳腺癌 细胞角蛋白19 上皮细胞黏附分子 免疫磁珠 

分 类 号：R737.9[医药卫生—肿瘤]