出芽短梗霉菌株PA-2全基因组测序及分析  

Complete genome sequencing and analysis of Aureobasidium pullulans strain PA-2

作　　者：高汉峰 刘晓芳 程亮 朱海霞 杨琼英 张纲 刘玉玲 郭青云 GAO Hanfeng;LIU Xiaofang;CHENG Liang;ZHU Haixia;YANG Qiongying;ZHANG Gang;LIU Yuling;GUO Qingyun(Institute of Plant Protection,Qinghai Academy of Agriculture and Forestry Science,Qinghai University,Xining,Qinghai 810016,China;Ledu District Agricultural Technology Extension Center of Haidong City,Haidong,Qinghai 810799,China)

机构地区：[1]青海大学农林科学院植物保护研究所,青海西宁810016 [2]海东市乐都区农业技术推广中心,青海海东810799

出　　处：《微生物学通报》2021年第9期3002-3012,共11页Microbiology China

基　　金：国家自然科学基金(31760539);青海省农业有害生物综合治理重点实验室开放基金(2021-ZJ-Y08)。

摘　　要：【背景】出芽短梗霉菌株PA-2是一株分离自青海省海东市平安区患病杨树叶片上的真菌,前期研究表明该菌株具有除草和抑菌能力,说明其在生物农药方面具有潜在的应用前景。【目的】了解菌株PA-2的基因组序列信息,挖掘其生防相关功能基因簇,为进一步研究解析该菌株生防机理及生防功能改造提供遗传背景信息。【方法】利用IlluminaHiSeq高通量测序平台对生防菌株PA-2进行全基因组测序,用生物信息学的方法对测序数据进行基因组组装、基因预测及功能注释、碳水化合物活性酶预测、次级代谢产物合成基因簇预测,利用刚果红染色等方法对水解酶活性进行衡量。【结果】菌株PA-2基因组序列全长28932793 bp,平均GC含量为50%,共编码10839个基因,预测到该菌株具有4个已知的次级代谢产物合成基因簇,编码Melanin、Burnettramic Acid A、ACR-Toxin I、Abscisic Acid,该菌株能水解纤维素和果胶。【结论】有助于在基因组层面上解析菌株PA-2生防机制的内在原因,为深入了解出芽短梗霉菌次级代谢物合成途径提供参考,对菌株PA-2的下一步相关研究具有重要意义。[Background]Aureobasidium pullulans PA-2 is a fungal strain isolated from the infected poplar leaves in Ping’an district,Haidong city of Qinghai province,China.Previous studies demonstrated that this strain showed herbicidal and antimicrobial activities,suggesting that it may have potential in biological pesticide.[Objective]To understand the information of genome sequence of A.pullulans strain PA-2 and mine gene clusters related to its biocontrol function.These results provide genetic basic information in the future to analyze its biocontrol mechanism and functional modification.[Methods]Based on Illumina HiSeq high-throughput sequencing platform,A.pullulans strain PA-2 was sequenced.Bioinformatics methods were used to genome assembly,gene prediction,functional annotation,prediction of carbohydrate active enzymes and secondary metabolite synthetic gene clusters.Hydrolase activity was measured by Congo red staining and other methods.[Results]The size of the genome is 28932793 bp with an average of GC content at 50%,which might code the potential 10839 genes.At the same time,4 known secondary metabolite synthetic gene clusters were also predicted,which might code the melanin,burnettramic acid A,ACR-toxin I and abscisic acid.The strain can hydrolyze cellulose and pectin.[Conclusion]This study provides useful information to analyze the internal causes of the biocontrol mechanism of strain PA-2 at the genomic level,and offers reference for further understanding of secondary metabolite synthetic pathways of A.pullulans.It is of great significance for further studies involved in strain PA-2.

关 键 词：出芽短梗霉菌 全基因组测序 水解酶活性 次级代谢产物 生物防治 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]