QuEChERS-超高效液相色谱-串联质谱法测定2种茄果类有机蔬菜中三唑酮及其代谢物  

Determination of triadimefon and its metabolites in 2 kinds of solanaceous organic vegetables by QuEChERS-ulra performance liquid chromatographytandem mass spectrometry

作　　者：刘庆菊 陈婉 韩平 LIU Qing-Ju;CHEN Wan;HAN Ping(Beijing Research Center for Agriculture Standards and Testing,Beijing 100097,China;Risk Assessment Lab for Agro-Products(Beijing),Ministry of Agriculture,Beijing 100097,China)

机构地区：[1]北京农业质量标准与检测技术研究中心,北京100097 [2]农业部农产品质量安全风险评估实验室(北京),北京100097

出　　处：《食品安全质量检测学报》2021年第16期6457-6464,共8页Journal of Food Safety and Quality

基　　金：北京市农林科学院青年科研基金项目(QNJJ202114);20192020年国家农产品质量安全风险评估项目(GJFP20190011)。

摘　　要：目的建立QuEChERS结合超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)测定2种茄果类蔬菜中三唑酮及其代谢产物三唑醇对映异构体的分析方法。方法采用乙腈作为提取溶剂,N-丙基乙二胺(primary secondary amine,PSA)混合石墨化碳黑净化,样品经CHIRAL?OD-RH手性柱分离,采用UPLC-MS/MS测定,基质外标法定量。结果三唑酮和三唑醇在0.02~1.00 mg/L范围内线性良好,相关系数均在0.99以上,定量限(limits of quantitation,LOQ)为0.02 mg/L。有机茄子、有机青椒中3个添加水平的平均回收率为71.0%~97.8%,相对标准偏差(relative standard deviation,RSD)(n=6)在1.2%~9.9%之间。结论该方法操作简便,准确度高、成本低,可以用于测定茄果类有机蔬菜中三唑酮及代谢物三唑醇残留。Objective To establish a method for the determination of triadimefon and its metabolite triadimenol enantiomers in 2 kinds of solanaceous vegetables by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods Acetonitrile was used as the extraction solvent,primary secondary amine(PSA)was mixed with graphitized carbon black and the samples were purified by CHIRAL?OD-RH chiral column separation,determined by UPLC-MS/MS and quantified by matrix external standard method.Results The linear range for triadimefon and triadimenol were 0.02-1.00 mg/L,the correlation coefficients were all above 0.99,and limits of quantitation(LOD)was 0.02 mg/L.The average recoveries rate of the 3 addition levels in organic eggplant and organic green pepper were 71.0%-97.8%,and the relative standard deviations(RSDs)(n=6)were 1.2%-9.9%.Conclusion The method is simple to operate,has high accuracy and low cost,and can be used to determine triadimefon and metabolite residues in solanaceous organic vegetables.

关 键 词：QUECHERS 超高液相色谱-串联质谱法 三唑酮 三唑醇 茄果类有机蔬菜 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]