miR-181c-5p靶向作用Beclin1抑制口腔鳞癌SCC-090细胞增殖并促进细胞凋亡的机制  

The effects of miR-181c-5p in inhibiting proliferation and promoting apoptosis of oral squamous cell carcinomas SCC-090 cell by targetting Beclin1

作　　者：刘军刚 刘洋[2] 哈庆 LIU Jungang;LIU Yang;HA Qing(Department of Stomatology,Beijing Rehabilitation Hospital Affiliated to Capital Medical University,Beijing 100144,China;不详)

机构地区：[1]首都医科大学附属北京康复医院口腔科,北京市100144 [2]北京大学口腔医院第三门诊部 [3]北京大学口腔医院急诊科

出　　处：《河北医药》2021年第18期2734-2737,共4页Hebei Medical Journal

摘　　要：目的检测雷帕霉素刺激SCC-090后miR-181c-5p的表达量,验证后者与自噬基因Beclin1的作用关系及对SCC-090的增殖与凋亡的影响。方法应用qRT-PCR检测雷帕霉素(RAPA)及3-甲基腺嘌呤(3-MA)分别刺激SCC-090细胞后miR-181c-5p的表达量;应用qRT-PCR、免疫印迹及荧光活性验证miR-181c-5p与Beclin1的作用关系;应用CCK8检测细胞的增殖,应用流式细胞术检测凋亡细胞比率,应用Western blot检测凋亡基因Bcl-2的表达。结果雷帕霉素处理细胞后,miR-181c-5p表达升高(P<0.05),3-MA处理后的细胞中,miR-181c-5p的表达下降(P<0.05);当miR-181c-5p高表达时,Beclin1表达下调(P<0.05),当miR-181c-5p表达降低时,Beclin1表达升高(P<0.05);miR-181c-5p与Beclin1有靶向关系;miR-181c-5p可明显抑制SCC-090细胞的增殖并促进细胞的凋亡。结论雷帕霉素诱导SCC-090细胞自噬后miR-181c-5p的表达量明显升高,且miR-181c-5p可通过抑制Beclin1的表达降低SCC-090细胞的增值能力且促进细胞的凋亡。Objective To investigate the expression of miR-181c-5p of in SCC-090 after rapamycin(RAPA)stimulus,and to verify the correlation between the latter and the autophagy gene Beclin1.Methods The expression levels of miR-181c-5p in SCC-090 cells stimulated by RAPA and 3-methyl adenine(3-MA)respectively were detected by qRT-PCR.Western Blot and luciferase activity assay were used to verify the correlation between miR-181c-5p and Beclin1.The proliferation of SCC-090 cells was detected by CCK8,and the apoptotic rate was detected by Flow cytometry,and the expression levels of Bcl-2 were detected by Western Blot.Results After RAPA treatment,the expression levels of miR-181c-5p were significantly increased,however,after 3-MA treatment,which were were significantly decreased(P<0.05).When the expression levels of miR-181c-5p were increased,the expression levels of Beclin1 were decreased,when the expression levels of miR-181c-5p were decreased,the expression levels of Beclin1 were increased.There was a target correlation between miR-181c-5p and Beclin1,and miR-181c-5p could obviously inhibit the growth and promote the apoptosis of SCC-090 cells.Conclusion The expression levels of miR-181c-5p are increased significantly after autophagy of SCC-090 cells induced by RAPA,and miR-181c-5p can reduce cell proliferation and promote apoptosis of SCC-090 cells by inhibiting the expression of Beclin1.

关 键 词：口腔鳞癌 miR-181c-5p 自噬 BECLIN1 

分 类 号：R739.85[医药卫生—肿瘤]