lncRNA MIR205HG通过调控miR-122-5p促进食管鳞状细胞癌细胞的增殖和侵袭  

lncRNA MIR205HG promotes proliferation and invasion of esophageal squamous cell carcinoma cells by regulating miR-122-5p

作　　者：王继振 孙振峰 赵永强 刘公哲 马文慧 亓磊[3] WANG Jizhen;SUN Zhenfeng;ZHAO Yongqiang;LIU Gongzhe;MA Wenhui;QI Lei(Department of Thoracic Surgery,Jinan People's Hospital Affiliated to Shandong First Medical University,Shandong Jinan 271199,China;Department of Spleen,Stomach,Hepatobiliary,Jinan Hospital of Integrated Traditional Chinese and Western Medicine,Shandong Jinan 271199,China;Department of Thoracic Surgery,Qilu Hospital,Shandong University,Shandong Jinan 250012,China)

机构地区：[1]山东第一医科大学附属济南人民医院胸心外科,山东济南271199 [2]济南市中西医结合医院脾胃肝胆科,山东济南271199 [3]山东大学齐鲁医院胸外科,山东济南250012

出　　处：《现代肿瘤医学》2021年第19期3325-3330,共6页Journal of Modern Oncology

基　　金：山东省自然科学基金(编号:ZR2014HM072);山东省科学技术厅重点研究项目(编号:2019ZD0140)。

摘　　要：目的:探究长链非编码RNA(lncRNA)MIR205HG对食管鳞状细胞癌细胞增殖和侵袭的影响及其作用机制。方法:RT-PCR检测食管鳞状细胞癌组织以及癌旁组织中MIR205HG的表达量;CCK-8实验检测MIR205HG对食管鳞状细胞癌细胞Eca-109和TE-10增殖的影响;Western blot实验检测侵袭相关蛋白MMP-1和MMP-9以及血管内皮生长因子(VEGF)的蛋白表达水平。结果:结果显示,MIR205HG在食管鳞状细胞癌组织中的表达量显著高于癌旁组织的表达量(P<0.05);与转染对照si-RNA相比,当细胞转染si-MIR205HG时,MIR205HG的表达量显著被抑制(P<0.05);干扰MIR205HG显著抑制食管鳞状细胞癌细胞Eca-109和TE-10的增殖和侵袭(P<0.05);此外,si-MIR205HG促进miR-122-5p的表达,且MIR205HG与miR-122-5p表达量呈负相关(P<0.05);进一步的研究结果表明,抑制miR-122-5p逆转si-MIR205HG对TE-10细胞增殖和侵袭的抑制作用(P<0.05)。结论:MIR205HG能够促进食管鳞状细胞癌细胞的增殖和侵袭,其作用机制是通过调控miR-122-5p来实现的,这一结果能够为食管鳞状细胞癌的临床治疗和诊断提供分子基础。Objective:To investigate the effect of MIR205HG on the proliferation and invasion of esophageal squamous cell carcinoma cells and its mechanism.Methods:RT-PCR was used to detect the expression of MIR205HG in esophageal cancer and adjacent tissues.CCK-8 was used to detect the effect of MIR205HG on the proliferation of Eca-109 and TE-10 cells.Western blot was used to detect the expression of MMP-1,MMP-9 and VEGF.Results:The expression of MIR205HG in esophageal cancer was significantly higher than that in adjacent tissues(P<0.05).Compared with the control si-RNA,the expression of MIR205HG was significantly inhibited(P<0.05)when the cells were transfected with si-MIR205HG.The interference of MIR205HG significantly inhibited the proliferation and invasion of Eca-109 and TE-10(P<0.05).In addition,si-MIR205HG promoted the proliferation and invasion of esophageal cancer cells,and further study showed that inhibition of miR-122-5p reversed the inhibition of si-MIR205HG on the proliferation and invasion of TE-10 cells(P<0.05).Conclusion:MIR205HG promotes the proliferation and invasion of esophageal cancer cells by regulating miR-122-5p.

关 键 词：MIR205HG 食管鳞状细胞癌 miR-122-5p 增殖 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]