转录调节因子c-Myb通过炎症因子Ccl2抑制乳腺癌肺转移的实验研究  

Experimental research of c-Myb inhibiting lung metastasis of breast cancer by inflammatory factor Ccl2

作　　者：吕佰瑞[1] 尹培 张晓慧[3] 赵剑平[1] 刘春海[1] 霍红旗[1] LYU Bairui;YIN Pei;ZHANG Xiaohui;ZHAO Jianping;LIU Chunhai;HUO Hongqi(Department of Nuclear Medicine,Handan Central Hospital,Hebei Handan 056001,China;Department of Nuclear Medicine,the First Hospital of Hebei Medical University,Hebei Shijiazhuang 050031,China;Department of Oncology,Handan Central Hospital,Hebei Handan 056001,China)

机构地区：[1]邯郸市中心医院核医学科,河北邯郸056001 [2]河北医科大学第一医院核医学科,河北石家庄050031 [3]邯郸市中心医院肿瘤一科,河北邯郸056001

出　　处：《现代肿瘤医学》2021年第19期3347-3350,共4页Journal of Modern Oncology

基　　金：河北省卫健委医学研究课题(编号:20191861)。

摘　　要：目的:研究乳腺癌细胞中转录调节因子c-Myb的表达对肺转移的影响及其分子机制。方法:构建c-Myb高表达的4T1乳腺癌细胞株,种植小鼠构建乳腺癌动物模型,检测实验动物肺转移情况,通过荷瘤抑制试验检测肺转移抑制效果。提取组织进行荧光PCR检测相关炎症因子的表达。利用Medisapiens数据库的生物信息学资源,对c-Myb和Ccl2的表达与乳腺癌患者的预后进行分析。结果:高表达c-Myb的荷瘤小鼠肺部转移灶明显减少,其中炎症相关因子表达受限。由数据库分析得出,c-Myb高表达患者生存期得以延长。结论:乳腺癌中c-Myb的表达能够通过炎症因子Ccl2抑制肺转移从而延长患者生存期。Objective:To investigate the effect of c-Myb expression in breast cancer on lung metastasis and its molecular mechanism.Methods:c-Myb positive 4T1 breast cancer cell line was constructed,and the animal model of breast cancer was established in mice.Detect the lung metastasis of experimental animals,and the inhibition effect of lung metastasis was detected by tumor-bearing inhibition test.The tissues were extracted for fluorescence PCR detection to detect the expression of related inflammatory factors.Using bioinformatics analysis of Medisapiens database,the prognosis of breast cancer patients with c-Myb and Ccl2 expression were analysed.Results:The lung metastasis of tumor-bearing mice with high expression of c-Myb was significantly reduced,in which the expression of inflammation related factors was limited,and the survival time of patients was prolonged.Conclusion:The expression of c-Myb in breast cancer can prolong the survival of patients by inhibiting pulmonary metastasis through inflammatory factor Ccl2.

关 键 词：乳腺癌 C-MYB 炎症因子 肺转移 

分 类 号：R737.9[医药卫生—肿瘤]