油酸激活Src基因诱导Hela细胞增殖、迁移和侵袭的研究  

Study on oleic acid inducing Hela cell proliferation,migration and invasion by activating Src gene

作　　者：秦虹[1,2] 张晓玉 罗旋 曾晗 陈压西 杨萍 QIN Hong;ZHANG Xiaoyu;LUO Xuan;ZENG Han;CHEN Yaxi;YANG Ping(Oncology Center,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 401336,China;Centre for Lipid Research,Chongqing Key Laboratory of Lipid and Glucose Metabolism,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第二医院肿瘤中心,401336 [2]重庆医科大学脂糖代谢性疾病重庆市重点实验室脂质研究中心,400016

出　　处：《重庆医学》2021年第18期3084-3089,共6页Chongqing medicine

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0205);重庆市渝中区基础研究与前沿探索项目(20190120)。

摘　　要：目的探讨油酸是否能够通过激活Src基因,从而诱导宫颈癌Hela细胞的增殖、迁移和侵袭。方法给予不同浓度的油酸(0、5、10μmol/L)处理人宫颈癌Hela细胞,使用CCK-8、平板克隆、EdU及Transwell等方法分别检测不同浓度油酸负荷下Hela细胞增殖、迁移及侵袭能力的变化情况;Western blot检测油酸处理前后Src和p-Src蛋白表达水平变化;使用Src激酶特异性抑制剂SU6656处理细胞后,观察Hela细胞增殖率及穿膜细胞数等指标变化。结果不同浓度油酸处理Hela细胞后,其体外增殖、迁移和侵袭能力均明显增加(P<0.05),且存在浓度依赖性。与NC组比较,油酸组p-Src蛋白表达水平升高(P<0.05)。应用Src特异性抑制剂SU6656处理后,油酸引起的Hela细胞的增殖、迁移及侵袭力的增强均受到抑制。结论油酸激活Src基因促进Hela细胞增殖、迁移和侵袭。Objective To investigate whether oleic acid can induce cervical cancer Hela cell proliferation,migration and invasion by activating Src gene.Methods Hela cells were treated with different concentrations of oleic acid(0,5,10μmol/L).CCK-8,plate cloning,EdU and Transwell were respectively used to detect the ability of proliferation,migration and invasion of Hela cells with different oleic acid concentrations.Western blot was used to detect the Src and p-Src protein expression levels with oleic acid treatment.Then Src kinase-specific inhibitor SU6656 was used to treat Hela cells.CCK-8 and Transwell assay were conducted to observe the changes of proliferation rate and number of transmembrane cells in each group.Results After Hela cells were treated with oleic acid at different concentrations,the proliferation,migration and invasion rate in vitro increased significantly(P<0.05),and there was a concentration dependence.Compared with the control group,the expression level of p-src protein was increased in the oleic acid treatment group(P<0.05).At SU6656 pretreatment,the proliferation,migration and invasion of Hela cells induced by oleic acid were inhibited.Conclusion Oleic acid activates the Src gene to promote the proliferation,migration and invasion of Hela cells.

关 键 词：宫颈癌 HELA细胞 油酸 SRC SU6656 

分 类 号：R737.3[医药卫生—肿瘤]