sEH抑制剂激活EPCs促进梗死心肌Ⅷ因子生成的研究  

Study on sEH inhibitor activating EPCs to promote factorⅧproduction in infarcted myocardium

作　　者：王振河[1,2] 姜德谦[2] 许丹焰[2] WANG Zhenhe;JIANG Deqian;XU Danyan(Department of Cardiology,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian 361003,China;Department of Cardiology,Second Xiangya Hospital,Central South University,Changsha,Hunan 410011,China)

机构地区：[1]厦门大学附属第一医院心内科,福建厦门361003 [2]中南大学湘雅二医院心内科,长沙410011

出　　处：《重庆医学》2021年第18期3095-3101,共7页Chongqing medicine

基　　金：福建省卫生厅青年项目(2013-2-84)。

摘　　要：目的研究可溶性环氧化物水解酶(sEH)抑制剂t-AUCB干预的内皮祖细胞(EPCs)对小鼠梗死心肌边缘区Ⅷ因子生成的促进作用。方法冲洗小鼠四肢长骨骨髓,离心,分离,培养获取EPCs;不同浓度t-AUCB及t-AUCB+过氧化物酶增殖体激活受体-γ(PPAR-γ)阻断剂GW9662干预EPCs;开胸,分离心包,结扎小鼠左前降支近端,心电图检查明确心肌梗死;1 h后尾静脉注射200μL预先干预的EPCs,注射后1、3、7、14、28 d测定梗死心肌边缘区Ⅷ因子表达情况。结果注射后随着干预时间延长及t-AUCB干预浓度增加,梗死心肌边缘区Ⅷ因子表达逐渐增多,给予PPAR-γ抑制剂GW9662后,Ⅷ因子表达明显减少,但仍多于未干预组(P<0.05)。结论sEH抑制剂可明显激活EPCs促进梗死心肌边缘区Ⅷ因子生成作用,PPAR-γ通路被激活与上述作用有关。Objective To research the function of soluble epoxide hydrolase(sEH)inhibitors t-AUCB intervention of endothelial progenitor cells(EPCs)promoting infarction myocardial margin factorⅧgenerate in mice.Methods The long bone marrow of mice limbs was washed,centrifuged,isolated and cultured to obtain endothelial progenitor cells.Different concentrations of t-AUCB and t-AUCB+peroxisome proliferator activated receptor-γ(PPAR-γ)inhibitor GW9662 interfered with endothelial progenitor cells.After thoracotomy,pericardium was separated,the proximal left anterior descending branch of the mice was ligated,and the myocardial infarction was confirmed by electrocardiogram.After 1 h,200μL of pre-intervention endothelial progenitor cells were injected into the tail vein,detected factorⅧexpression of infarction myocardial margin at postoperative 1,3,7,14,28 days.Results After transplantation with prolonged intervention and t-AUCB intervention concentration increasing,factorⅧexpression gradually increased in infarction myocardial margin,while factorⅧexpression decreased significantly after giving PPAR-γinhibitor GW9662,but which was still more than that in the non-intervention group(P<0.05).Conclusion Soluble epoxide hydrolase inhibitors can obviously activated endothelial progenitor cells promote infarction myocardial margin factorⅧgenerated,ppar-gamma pathways activated related to the role.

关 键 词：可溶性环氧化物水解酶抑制剂 过氧化物酶增殖体激活受体-γ 内皮祖细胞 心肌梗死 Ⅷ因子 

分 类 号：R361[医药卫生—病理学]