子宫内膜样癌中SIAH2的表达及临床意义  

Expression and clinical significance of SIAH2 in endometrioid carcinoma

作　　者：甄娟[1] 刘玉东 宋春红[1] 吴莎 焦瑞芬 ZHEN Juan;LIU Yu-dong;SONG Chun-hong;WU Sha;JIAO Rui-fen(Department of Pathology,Shijiazhuang Fourth Hospital/Obstetrics and Gynecology Hospital Affiliated to Hebei Medical University,Shijiazhuang 050011,China;Department of Otolaryngology,Hebei General Hospital,Shijiazhuang 050057,China;Department of Obstetrics and Gynecology,Shijiazhuang Fourth Hospital/Obstetrics and Gynecology Hospital Affiliated to Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北省石家庄市第四医院/河北医科大学附属妇产医院病理科,石家庄050011 [2]河北省人民医院耳鼻咽喉科,石家庄050057 [3]河北省石家庄市第四医院/河北医科大学附属妇产医院妇产科,石家庄050011

出　　处：《临床与实验病理学杂志》2021年第7期809-813,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：石家庄市科学技术研究与发展指导计划(171462003)、河北省医学科学研究重点课题计划(20181070)。

摘　　要：目的探讨癌旁正常子宫内膜、子宫内膜非典型增生及子宫内膜样癌组织中SIAH2的表达及其与子宫内膜样癌临床病理特征的关系。方法采用免疫组化SP法检测癌旁正常子宫内膜、子宫内膜非典型增生及子宫内膜样癌组织中SIAH2的表达;采用qRT-PCR和Western blot法检测新鲜子宫内膜组织中SIAH2 mRNA和蛋白的表达。利用Kaplan-Meier法绘制生存曲线,Log-rank法分析SIAH2表达与子宫内膜样癌患者预后的关系。Cox比例风险回归模型筛选子宫内膜样癌患者预后的独立预测因素。结果免疫组化结果表明,在癌旁正常子宫内膜、子宫内膜非典型增生和子宫内膜样癌中,SIAH2的阳性率逐渐升高,分别为7.50%、42.00%和74.14%,差异有统计学意义(P<0.05)。SIAH2与子宫内膜样癌FIGO分期、肌层浸润深度和淋巴结转移相关(P均<0.05)。qRT-PCR和Western blot结果均显示,子宫内膜样癌组织中SIAH2 mRNA和蛋白的相对表达量与癌旁正常子宫内膜相比,均明显升高,差异有统计学意义(P均<0.05)。生存分析发现,SIAH2阳性子宫内膜样癌患者的生存时间明显短于阴性患者(P<0.05)。Cox多因素回归分析表明,SIAH2高表达是子宫内膜样癌患者总生存期的独立预测因子。结论SIAH2在子宫内膜样癌发生、发展中异常表达,SIAH2阳性的子宫内膜样癌更易发生淋巴结转移,并且与患者的不良预后有关,SIAH2有望成为子宫内膜样癌靶向治疗和预后评估的一项指标。Purpose To investigate the expression of SIAH2 in paracancerous normal endometrium,atypical endometrial hyperplasia and endometrioid carcinoma,and its relationship with clinicopathological parameters of endometrioid carcinoma.Methods The expression of SIAH2 in paracancerous normal endometrium,atypical endometrial hyperplasia and endometrioid carcinoma was determined by immunohistochemical SP method.The expression of SIAH2 mRNA and protein in fresh cenometrial cancer tissues was detected by qRT-PCR and Western blot.Survival curves were calculated using the Kaplan-Meier method and the relationship between the expression of SIAH2 and the prognosis of endometrioid carcinoma was analyzed by the Log-rank test.Cox proportional hazards regression model was used to examine the independent predictive factors of the prognosis of patients with endometrioid carcinoma.Results The immunohistochemistry results showed there was an increasing tendency in positive rate of SIAH2 expression from paracancerous normal endometrium(7.50%)to atypical endometrial hyperplasia(42.00%)to endometrial carcinoma(74.14%),and the difference was statistically significant(P<0.05).The expression of SIAH2 was correlated with FIGO stage,myometrial invasion and lymphatic metastasis(all P<0.05).qRT-PCR and Western blot showed that the relative expression of SIAH2 mRNA and protein were significantly increased from the paracancerous normal endometrium to endometrioid carcinoma,and there was significant difference(both P<0.05).Survival analysis showed that the survival time of endometrioid carcinoma patients with high SIAH2 expression was lower than that with low SIAH2 expression(P<0.05).Cox the multiplefactor hazards regression analysis revealed that the higher expression of SIAH2 was an independent prognostic factor for overall survival of endometrioid carcinoma.Conclusion The expression of SIAH2 is abnormal expressed in the development of endometrioid carcinogenesis,the patients of endometrioid carcinoma with SIAH2 positive expression is more prone to ly

关 键 词：子宫肿瘤 内膜样癌 SIAH2 临床病理因素 预后 

分 类 号：R737.33[医药卫生—肿瘤]