Mutation of the PHYTOENE DESATURASE 3 gene causes yellowish-white petals in Brassica napus  

作　　者：Chuanji Zhao Luqman Bin Safdar Meili Xie Meijuan Shi Zhixue Dong Li Yang Xiaohui Cheng Yueying Liu Zetao Bai Yang Xiang Chaobo Tong Junyan Huang Lijiang Liu Shengyi Liu 

机构地区：[1]Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/The Key Laboratory of Biology and Genetic Improvement of Oil Crops,The Ministry of Agriculture and Rural Affairs,Wuhan 430062,Hubei,China [2]National Key Laboratory of Crop Genetic Improvement,National Center of Rapeseed Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China [3]Biosystematics Group,Experimental Plant Sciences,Wageningen University and Research,Wageningen,Netherlands [4]Guizhou Rapeseed Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550008,Guizhou,China

出　　处：《The Crop Journal》2021年第5期1124-1134,共11页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program Of China (2016YFD0101007 and 2018YFE0108000);National Natural Science Foundation of China (31770250);the Natural Science Foundation of Hubei Province (2019CFB628);China Agriculture Research System (CARS-12);Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences;The Agricultural Scientific and Technological Research Projects of Guizhou Province (No. Qiankehezhicheng [2019] 2397)。

摘　　要：Oilseed rape (Brassica napus) with yellow flowers is an attractive ornamental landscape plant during the flowering period,and the development of different petal colors has become a breeding objective.Although yellowish flower color is a common variant observed in field-grown oilseed rape,the genetics behind this variation remains unclear.We obtained a yellowish-white flower (ywf) mutant from Zhongshuang 9 (ZS9) by ethyl methanesulfonate mutagenesis (EMS) treatment.Compared with ZS9,ywf exhibited a lower carotenoid content with a reduced and defective chromoplast ultrastructure in the petals.Genetic analysis revealed that the yellowish-white trait was controlled by a single recessive gene.Using bulked-segregant analysis sequencing (BSA-seq) and kompetitive allele-specific PCR(KASP),we performed map-based cloning of the ywf locus on chromosome A08 and found that ywf harbored a C-to-T substitution in the coding region,resulting in a premature translation termination.YWF,encoding phytoene desaturase 3 (PDS3),was highly expressed in oilseed rape petals and involved in carotenoid biosynthesis.Pathway enrichment analysis of the transcriptome profiles from ZS9 and ywf indicated the carotenoid biosynthesis pathway to be highly enriched.Further analyses of differentially expressed genes and carotenoid components revealed that the truncated Bna A08.PDS3 resulted in decreased carotenoid biosynthesis in the mutant.These results contribute to an understanding of the carotenoid biosynthesis pathway and manipulation of flower-color variation in B.napus.

关 键 词：Yellowish-white flower BSA-seq RNA-SEQ BnaA08.PDS3 Carotenoid biosynthesis 

分 类 号：S565.4[农业科学—作物学]