依普黄酮对骨质疏松大鼠颌骨成骨细胞ER和PCNA表达的影响  

Effects of ipriflavone on the expression of ER and PCNA in osteoblasts from the jaws of osteoporotic rats

作　　者：于书娟 刘洪臣[2] YU Shu-juan;LIU Hong-chen(Stomatology Department,960th Hospital of PLA,Shandong 250031,China;In-stitute of Stomatology&Oral Maxilla Facial Key Laboratory,Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]中国人民解放军第九六〇医院口腔科,山东250031 [2]中国人民解放军总医院口腔医学研究所,口腔颌面战创伤军队重点实验室,口腔颌面修复军队医学重点实验室,北京100853

出　　处：《口腔颌面修复学杂志》2021年第5期321-325,共5页Chinese Journal of Prosthodontics

摘　　要：目的:研究雌激素受体(ER)和增殖细胞核抗原(PCNA)在依普黄酮作用于骨质疏松大鼠颌骨成骨细胞时的表达情况。方法:SD大鼠骨质疏松动物模型通过双侧卵巢切除术建立。取下颌骨成功培养成骨细胞,含10-8M依普黄酮的培养基干预培养成骨细胞3天,通过RT-PCR检测ERα、ERβ、PCNA的mRNA表达。结果:10-8M浓度的依普黄酮可以促进PCNA和ERβ的mRNA的表达,抑制ERα的mRNA的表达。结论:依普黄酮可能通过增加ERβ、PCNA和降低ERα的表达,对骨质疏松大鼠颌骨成骨细胞起调控作用。Objective:The objective of this study was to identify the effeets of ipriflavonc on thc expression ofcstrogcn receptor(ER)and proliferating cell nuclear antigen(PCNA)in ostcoblasts derived from thc jaws ofostcoporotic rats.Methods:An ostcoporotic animal model was cstablished by performing a bilatcral ovaricctomy.Then ostcoblasts from the jaws of ostcoporotic rats were cultured in the medium containing ipriflavonc(10^(-8) M)for72 h.The mRNA cxpressions of ERa,ERβ,and PCNA were detected by RT-PCR.Results:lpriflavonc at 10^(-8) Mconcentration inhibited thc expression of ERa mRNA and promotcd thc cxprcssions of PCNA and ERβmRNA.Conclusion:Ipriflavonc may play a regulatory rolce on ostcoblasts of jaws in ostcoporotic rats by incrcasing ERβ,PCNA and decreasing ERa mRNA.

关 键 词：成骨细胞 ER 骨质疏松 PCNA 依普黄酮 

分 类 号：R780.2[医药卫生—口腔医学]