PinX1调控PI3K/Akt信号通路影响口腔鳞癌HSC6细胞的增殖、侵袭和凋亡  

PinX1 affects the proliferation,invasion and apoptosis of oral squamous cell carcinoma HSC6 cells via PI3K/Akt signaling pathway

作　　者：邓璐[1] 曾利伟[1] 焦纪兰[1] 江辉[1] DENG Lu;ZENG Liwei;JIAO Jilan;JIANG Hui(First Department of Prosthodontics,The Affiliated Stomatological Hospital of Nanchang University,China,330006)

机构地区：[1]南昌大学附属口腔医院修复一科,江西省口腔生物医学重点实验室,330006

出　　处：《实用口腔医学杂志》2021年第5期700-705,共6页Journal of Practical Stomatology

基　　金：江西省教育厅科学技术研究项目-青年项目(编号:190161)。

摘　　要：目的:探讨人Pin2结合蛋白X1(PinX1)对口腔鳞癌(OSCC)HSC6细胞增殖、侵袭和凋亡的影响及其机制。方法:免疫组织化学方法检测Pin X1蛋白在人OSCC组织中的表达情况。免疫印迹法检测HSC6细胞和人正常口腔上皮HOEC细胞中PinX1蛋白的表达。CCK-8法、Transwell实验、流式细胞术检测过表达PinX1对HSC6细胞增殖、凋亡和侵袭的影响。免疫印迹法检测p-PI3K和p-Akt蛋白的表达。结果:人OSCC组织中Pin X1表达率低于癌旁组织(P<0.05)。HSC6细胞中PinX1蛋白表达低于HOEC细胞(P<0.05)。过表达PinX1后HSC6细胞活力、侵袭细胞数、p-PI3K和p-Akt蛋白表达显著降低(P<0.05),凋亡率显著升高(P<0.05)。激活PI3K/Akt信号通路能逆转PinX1过表达对HSC6细胞增殖、侵袭和凋亡的影响(P<0.05)。结论:PinX1通过抑制PI3K/Akt信号通路抑制口腔鳞癌HSC6细胞增殖、侵袭并促进其凋亡。Objective:To investigate the effects of human Pin2 binding protein X1(PinX1)on the proliferation,invasion and apoptosis of oral squamous cell carcinoma(OSCC)HSC6 cells and its mechanism.Methods:The expression of PinX1 in OSCC tissue was detected by immunobistochemical method,that in HSC6 cells and human oral epithelial HOEC cells was detected by Western blotting.CCK-8 assay,Transwell assay and flow cytometry were used to detect the effects of PinX1 overexpression on the proliferation,apoptosis and invasion of HSC6 cells.The expression of p-PI3K and p-Akt protein was assessed using Western blotting.Results:The expression rate of PinX1 protein in OSCC tissue was lower than that in adjacent tissue(P<0.05).The expression level of PinX1 protein in HSC6 cells was lower than that in HOEC cells(P<0.05).After overexpression of PinX1,the viability,invasion,p-PI3K and p-Akt protein expression of HSC6 cells were reduced(P<0.05),whereas the apoptosis rate was increased(P<0.05).Activation of the PI3K/Akt signaling pathway reversed the effects of PinX1 overexpression on proliferation,invasion and apoptosis of HSC6 cells(P<0.05).Conclusion:PinX1 inhibits proliferation,invasion and promotes apoptosis HSC6 cells by inhibiting PI3K/Akt signaling pathway.

关 键 词：口腔鳞癌 PinX1 细胞增殖 侵袭 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]