珍珠层粉复合富血小板纤维蛋白对颅骨缺损模型兔的修复作用  

Repair effect of nacre powder combined with platelet-rich fibrin on cranial defect model rabbits

作　　者：黄莹 马福娟 MAGED ALI Al-Aroomi 王触灵 谢富强[1,2] HUANG Ying;MA Fujuan;MAGED ALI Al-Aroomi;WANG Chuling;XIE Fuqiang(Department of Stomatology,Second Hospital,Lanzhou University,Lanzhou 730000,China;School of Stomatology,Lanzhou University,Lanzhou 730000,China;Department of Stomatology,Armed Police General Hospital of Gansu Province,Lanzhou 730000,China)

机构地区：[1]兰州大学第二医院口腔科,甘肃兰州730000 [2]兰州大学口腔医学院,甘肃兰州730000 [3]武警甘肃总队医院口腔科,甘肃兰州730000

出　　处：《吉林大学学报（医学版）》2021年第5期1162-1170,共9页Journal of Jilin University：Medicine Edition

基　　金：教育部中央高校基础研究基金项目(lzujbky2020-cd03);甘肃省科技厅自然科学基金项目(20JR10RA737)。

摘　　要：目的:制备珍珠层粉(NP)和富血小板纤维蛋白(PRF)的复合材料,分析其修复颅骨缺损模型兔效果,阐明其可能的作用机制。方法:选择12只新西兰兔建立兔颅骨缺损模型,颅骨顶部矢状缝周围3 cm×3 cm区域脱毛处理,全身麻醉后,于矢状缝两侧,造成4个直径8 mm的全层颅骨缺损孔,并保留完整的硬脑膜。每只新西兰兔4个颅骨缺损孔按照植入物不同分为4组,分别为空白对照组、NP组、PRF组和复合组,于4、8和12周各处死4只新西兰兔,取颅骨缺损标本,HE染色观察各组新西兰兔颅骨缺损部位骨组织病理形态表现,MicroCT扫描检测各组新西兰兔骨矿物质密度(BMD)和新生骨体积/骨总体积百分数(BV/TV),免疫组织化学法检测各组新西兰兔颅骨缺损部位新骨组织中骨形态发生蛋白2(BMP-2)和血管内皮生长因子(VEGF)蛋白表达水平。结果:4、8和12周观察,与空白对照组、NP组和PRF组比较,复合组新西兰兔大体标本成骨明显丰满。Micro CT扫描,与空白对照组、NP组和PRF组比较,4、8和12周时复合组新西兰兔颅骨缺损部位新骨形成面积更大,BMD和BV/TV明显升高(P<0.05),以第12周最为明显(P<0.05)。HE染色,4周时复合组新西兰兔颅骨缺损部位骨细胞、类骨质和新生血管较空白对照组、NP组和PRF组增多,8周时复合组新西兰兔颅骨缺损部位骨矿化现象及新生骨小梁较空白对照组、NP组和PRF组更加明显,12周时复合组新西兰兔颅骨缺损部位新生骨小梁增粗且成熟程度较空白对照组、NP组和PRF组高。免疫组织化学检测,4、8和12周时复合组新西兰兔颅骨缺损部位新骨组织中BMP-2和VEGF蛋白表达水平较空白对照组、NP组和RFP组均明显升高(P<0.05)。结论:NP复合PRF制备的复合材料有明显的促进骨组织生长能力,并且可能通过诱导BMP-2和VEGF的增加,促进骨组织的再生。Objective:To prepare the composite material of nacre powder(NP)and platelet-rich fibrin(PRF)and analyze its repair effect in the cranial defect model rabbits,and to clarify its possible mechanism.Methods:Twelve New Zealand rabbits were selected to set up the rabbit cranial defect models,the 3 cm×3 cm area around the sagittal suture at the top of the cranial was depilated.After general anesthesia,four full-thickness cranial defects with a diameter of 8 mm were created on both sides of the sagittal suture but the intact dura mater was retained.Four cranial defects of each New Zealand rabbit were divided into four groups according to different implants:blank control group(no implant material),NP group(only NP),PRF group(only PRF),and mixed group(NP+PRF).Four New Zealand rabbits were sacrificed at 4,8 and 12 weeks respectively,and the cranial defect specimens were collected.Micro CT scan was adopt to detect the bone mineral density(BDM)and the percentages of new bone volome/total bone volume(BV/TV).The pathomorphology of the bone in the cranial defect areas in various groups were observed with HE staining.The expression levels of bone morphogenetic protein-2(BMP-2)and vascular endothelial growth factor(VEGF)in the new bones of the New zealand rabbits in various groups were analysed by immunohistochemistry.Results:At 4,8 and 12 weeks,compared with blank control group,NP group and PRF group,the bone formation of the gross specimens in mixed group was significantly plump.The results of Micro CT scan showed that compared with blank control group,NP group and PRF group,at 4,8 and 12 weeks,a larger area of new bone was formed in mixed group,and BMD and BV/TV were significantly increased(P<0.05),especially at 12 weeks(P<0.05).The results of HE staining showed that the bone cells,osteoid,and new blood vessels of the New Zealand rabbits in mixed group at 4 weeks were higher than those in blank control group,NP group and PRF group;at 8 weeks,bone mineralization and neonatal bone trabecula of the New Zealand rabbits in mixed g

关 键 词：珍珠层粉 富血小板纤维蛋白 颅骨缺损 骨诱导 

分 类 号：R782[医药卫生—口腔医学]