10株宫颈癌细胞系中MALAT1的表达及其对顺铂敏感性的影响  

Expression of MALAT1 in 10 Cervical Cancer Cell Lines and Its Effect on Cisplatin Sensitivity

作　　者：张治洋 李功娟 ZHANG Zhi-yang;LI Gong-juan(Department of Gynecology,Ankang People’s Hospital,Shaanxi Ankang 725000,China;Department of Gynecology,the First Hospital of Hanbin District,Shaanxi Ankang 725000,China)

机构地区：[1]安康市人民医院妇科,陕西安康725000 [2]安康市汉滨区第一医院妇科,陕西安康725000

出　　处：《现代检验医学杂志》2021年第5期110-113,188,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探究肺腺癌转录相关转录本1(metastasis associated lung adenocarcinoma transcript 1,MALAT1)在10株宫颈癌(cervical cancer,CC)细胞中的表达及其对顺铂敏感性的影响。方法选取10株CC细胞系(BT-B,C-33A,CaSKi,MEG-01,Hela,HELA-S3,Ishikawa,RL95-2,HCC94和MS751),通过CCK-8实验验证MALAT1对10株宫颈癌细胞增殖的影响;通过化疗敏感性实验验证MALAT1对10种宫颈癌细胞化疗敏感性的影响;通过荧光素酶报告实验检测MALAT1野生质粒的荧光素酶活性。结果miR-126-5p在宫颈癌细胞株中的表达与MALAT1呈负相关(R=-0.673,P=0.033)。干扰MALAT1能够抑制宫颈癌细胞增殖(均P<0.05),干扰MALAT1能够增加宫颈癌细胞系对顺铂的敏感性(均P<0.05)。过表达miR-126-5p能够抑制宫颈癌细胞增殖(P<0.05),过表达miR-126-5p能够增加宫颈癌细胞系对顺铂的敏感性(P<0.05)。结论MALAT1在宫颈癌中能够通过靶向调控miR-126-5p的表达,进而调节宫颈癌细胞对顺铂的敏感性,进而导致宫颈癌患者对顺铂产生耐药。Objective To explore the expression and effect on the chemoresistance.Methods Detected the effect of metastasis associated lung adenocarcinoma transcript 1(MALAT1)on proliferation on 10 cervical cancer cells using CCK-8 assay,and deteced the effect of MALAT1 on sensitivity to chemotherapy on 10 cervical cancer cells using improved CCK-8 assay,Luciferase activity of MALAT1 wild plasmid was detected by luciferase reporter assay.Results The expression of miR-126-5p in cervical cancer cell line was negatively correlated with MALAT1(r=-0.673,P=0.033).Knockdown MALAT1 could inhibit the proliferation of cervical cancer cells(all P<0.05).Knockdown MALAT1 increased the sensitivity of cervical cancer cell lines to cisplatin(all P<0.05).Overexpression of miR-126-5p could inhibit the proliferation of cervical cancer cells(P<0.05).Over-expression of miR-126-5p could increase the sensitivity of cervical cancer cell lines to cisplatin(P<0.05).Conclusion MALAT1 can regulate the expression of miR-126-5p in cervical cancer,thereby regulating the sensitivity of cervical cancer cells to cisplatin,and thereby leading to cisplatin resistance in patients with cervical cancer.

关 键 词：宫颈癌 肺腺癌转录相关转录本1 顺铂 

分 类 号：R737.33[医药卫生—肿瘤] R730.43[医药卫生—临床医学]