结直肠腺癌中CD163^(+)巨噬细胞、CD8^(+)T细胞与微血管密度的相关性  

Relationship Among CD163 Macrophage,CD8T Cell,Microvessel Density and Clinicopathological Features in Patients with Colorectal Adenocarcinoma

作　　者：蔡秋莉 陈芸[1] 蔡昕怡[1] 刘英竹 江凡 张丽娟[1] CAI Qiu-li;CHEN Yun;CAI Xin-yi;LIU Ying-zhu;JIANG Fan;ZHANG Li-juan(The Third Affiliated Hospital of Kunming Medical University,Kunming 650100,China)

机构地区：[1]昆明医科大学第三附属医院,云南昆明650100

出　　处：《肿瘤学杂志》2021年第8期622-627,共6页Journal of Chinese Oncology

基　　金：云南省科技厅昆明医科大学应用基础研究联合专项(2017FE468-070)。

摘　　要：[目的]探讨结直肠腺癌中CD163^(+)巨噬细胞、CD8^(+)T细胞、微血管密度(MVD)的相关性及与患者临床病理因素的关系。[方法]通过免疫组化SP法检测61例结直肠腺癌癌组织及远癌组织中CD163^(+)巨噬细胞、CD8^(+)T细胞、CD34的表达水平,分析CD163^(+)巨噬细胞、CD8^(+)T细胞、MVD的相关性及与患者临床病理因素的关系。[结果]CD163、CD8、MVD在癌组织及远癌组织中的表达差异具有统计学意义(P均<0.001)。CD163与MVD的表达具有显著正相关性(r=0.615,P<0.001),CD8与MVD的表达具有负相关性(r=-0.320,P=0.012),CD8与CD163的表达具有显著负相关性(r=-0.370,P=0.003)。CD163^(+)巨噬细胞与结直肠腺癌分化程度、淋巴结转移、远处转移、TNM分期相关(P<0.05),MVD与结直肠腺癌肿瘤位置、分化程度、淋巴结转移、远处转移、TNM分期相关(P<0.05),CD8^(+)T细胞与患者性别、年龄、肿瘤位置、分化程度、TNM分期、淋巴转移、远处转移均无关(P>0.05)。[结论]联合靶向消除TAMs及促进CD8^(+)T细胞的活化与增殖可能对结直肠癌的抗血管生成治疗有一定意义。[Objective]To investigate the relationship among CD163^(+)macrophage,CD8^(+)T cell,microvessel density(MVD)and clinicopathological features in patients with colorectal adenocarcinoma.[Methods]The expression of CD163^(+)macrophage,CD8^(+)T cells,and CD34 in 61 colorectal adenocarcinoma cancer tissues and distant cancer tissues were detected by immunohistochemical SP method.The relationship among CD163^(+)macrophage,CD8^(+)T cells,MVD and clinicopathological features of patients were analyzed.[Results]The expressions of CD163,CD8 and MVD were significantly different between cancer tissues and distant cancer tissues(all P<0.001).CD163 was positively correlated with MVD(r=0.615,P<0.001),CD8 was negatively correlated with MVD(r=-0.320,P=0.012),and CD8 was significantly negatively correlated with the expression of CD163(r=-0.370,P=0.003).CD163^(+)was correlated with the degree of differentiation,lymphatic metastasis,distant metastasis,and TNM stage of colorectal adenocarcinoma(P<0.05).MVD was correlated with the location,degree of differentiation,lymphatic metastasis,distant metastasis,and TNM stage(P<0.05).CD8^(+)T was not corrected with gender,age,tumor location,differentiation degree,TNM stage,lymphatic metastasis,and distant metastasis in(P>0.05).[Conclusion]The study indicates that combined targeted elimination of tumor associated macrophages and promotion of CD8^(+)T cell activation and proliferation may have an effect on antiangiogenic therapy for colorectal cancer.

关 键 词：结直肠肿瘤 肿瘤相关巨噬细胞 CD8+T细胞 血管生成 

分 类 号：R735.34[医药卫生—肿瘤]