羟基红花黄色素A抑制人肝癌细胞系Huh7发生上皮-间质转化  

Hydroxysafflor yellow A inhibits epithelial-mesenchymal transition in hepatocellular carcinoma cell line Huh7

作　　者：吴娜 王东 李京敏 白咸勇 WU Na;WANG Dong;LI Jing-min;BAI Xian-yong(Department of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, China)

机构地区：[1]滨州医学院基础医学院组织学与胚胎学教研室,山东烟台264003

出　　处：《基础医学与临床》2021年第10期1423-1427,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81073123)。

摘　　要：目的研究羟基红花黄色素A(HSYA)对人肝癌细胞系Huh7迁移及侵袭的影响,并通过体内外实验探究其是否影响肝癌细胞上皮-间质转化(EMT)。方法体外培养Huh7细胞,分为对照组、HSYA 80和160μmol/L实验组,分别处理24 h,用transwell小室法和细胞划痕实验检测细胞侵袭和迁移能力;Western blot检测细胞vimentin和E-cadherin蛋白表达;建立Huh7细胞裸鼠皮下移植瘤模型,HE染色观察移植瘤和肝脏组织形态;免疫组化检测移植瘤组织TGF-β1、vimentin和E-cadherin表达情况。结果与对照组相比,HSYA实验组Huh7细胞迁移与侵袭能力明显下降,E-cadherin蛋白表达升高,vimentin蛋白表达下降(P<0.05);与移植组相比,HSYA实验组(2.25 mg/kg)肝内无肿瘤细胞转移,移植瘤组织出现肿瘤细胞坏死,E-cadherin表达升高,vimentin和TGF-β1表达下降(P<0.05)。结论HSYA通过抑制肝癌细胞发生EMT,抑制肝癌细胞的迁移及侵袭能力。Objective To investigate the effect of hydroxysafflor yellow A(HSYA)on the migration and invasion in Huh7 cells,and to explore whether HSYA affects the epithelial-mesenchymal transition(EMT)of liver cancer in vitro and in vivo.Methods Human Huh7 cells were cultured in and divided into control group,80 and 160μmol/L HSYA experimental groups and treated for 24 h respectively.Wound healing and Transwell assay were used to evaluate the migration and invasion of HCC cells.Western blot analysis was used to detect the expression of vimentin and E-cadherin.To establish a model of Huh7 nude mice with subcutaneous xenograft,the morphology of transplanted tumor and liver tissues in nude mice were observed under microscopy with H/E staining.The expression of TGF-β1,vimentin and E-cadherin in tumor tissues was detected by immuno-histochemistry.Results Compared with the control group,cell migration and invasion of HSYA experimental group were significantly decreased,E-cadherin expression was increased,and vimentin expression was decreased(P<0.05).Compared with the transplantation group,there was no tumor cell metastasis and tumor cell necrosis been found in the HSYA experimental group(2.25 mg/kg),expression of E-cadherin was increased and expression of vimentin as well as TGF-β1 was decreased(P<0.05).Conclusions HSYA inhibits migration and invasion of HCC cells through inhibiting the occurrence of EMT.

关 键 词：羟基红花黄色素A 肝癌 上皮-间质转化 TGF-Β1 

分 类 号：R735.7[医药卫生—肿瘤]